{"id":"fbb77222-481e-5472-bd87-1d986a18ef6c","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-human-nrf2-up","predicate":"increases_expression","statement":"Luteolin reduced NFE2L2-promoter methylation and increased Nrf2 expression in human HCT116 colorectal cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"31128b2c-f5c1-50d7-aa9f-9e13a428739d","mechanism_event_label":"The direction differs from the mouse-liver result.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"31128b2c-f5c1-50d7-aa9f-9e13a428739d","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-human-nrf2-up-event","event_type":"observed_relationship","label":"The direction differs from the mouse-liver result.","description":"Luteolin reduced NFE2L2-promoter methylation and increased Nrf2 expression in human HCT116 colorectal cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human colorectal cell culture and bisulfite sequencing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different model; not a proven contradiction or universal epigenetic reset.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The direction differs from the mouse-liver result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The dietary flavone luteolin epigenetically activates the Nrf2 pathway and blocks cell transformation in human colorectal cancer HCT116 cells. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30129150/ · DOI 10.1002/jcb.27275","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ef3c2f42-af68-51a0-8fc8-7e47e4c54997","evidence_kind":"source_excerpt","locator":"Lines 468-474","start_line":468,"end_line":474,"excerpt":"## luteolin-human-nrf2-up\nThe direction differs from the mouse-liver result.\nLuteolin reduced NFE2L2-promoter methylation and increased Nrf2 expression in human HCT116 colorectal cells.\nModel: Human colorectal cell culture and bisulfite sequencing.\nLimitations: Different model; not a proven contradiction or universal epigenetic reset.\nEvidence access: Primary abstract\nThe dietary flavone luteolin epigenetically activates the Nrf2 pathway and blocks cell transformation in human colorectal cancer HCT116 cells. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30129150/ · DOI 10.1002/jcb.27275","model_system":"Human colorectal cell culture and bisulfite sequencing.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}