{"id":"fb1526c3-980d-5591-9e90-c9e998c4173e","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-ferroptosis-rescue","predicate":"induces_inhibitor_sensitive","statement":"In human H358 and A549 cells, deferoxamine, ferrostatin-1 and liproxstatin-1 reversed cucurbitacin B growth inhibition, supporting a ferroptotic component.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c37c037b-bbbc-5342-af9c-521176f173b5","mechanism_event_label":"Iron chelation and lipid-radical inhibitors changed the outcome.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"cec30c19-2d02-5f08-9a38-a0fa2b49e22d","slug":"human-cucurbitacin-ferroptosis","display_name":"Human NSCLC ferroptotic response during cucurbitacin B exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c37c037b-bbbc-5342-af9c-521176f173b5","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-ferroptosis-rescue-event","event_type":"observed_relationship","label":"Iron chelation and lipid-radical inhibitors changed the outcome.","description":"In human H358 and A549 cells, deferoxamine, ferrostatin-1 and liproxstatin-1 reversed cucurbitacin B growth inhibition, supporting a ferroptotic component.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cec30c19-2d02-5f08-9a38-a0fa2b49e22d","slug":"human-cucurbitacin-ferroptosis","display_name":"Human NSCLC ferroptotic response during cucurbitacin B exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f305bdb9-9306-5aee-9039-4572f5ccbc67","slug":"ferrostatin-1","display_name":"Ferrostatin-1","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"279d00c2-a6a3-5943-8d5d-a7eed68e1041","slug":"deferoxamine","display_name":"Deferoxamine","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"48d83ef6-aca3-5ddc-af55-22fefd99a7f0","slug":"liproxstatin-1","display_name":"Liproxstatin-1","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human NSCLC cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rescue does not establish exclusive cell-death causation or clinical cancer efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Iron chelation and lipid-radical inhibitors changed the outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B targets STAT3 to induce ferroptosis in non-small cell lung cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38950838/ · DOI 10.1016/j.ejphar.2024.176805","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"83ed0f19-a25d-55fe-a120-412f8c30c8a3","evidence_kind":"source_excerpt","locator":"Lines 252-258","start_line":252,"end_line":258,"excerpt":"## cucurbitacin-b-ferroptosis-rescue\nIron chelation and lipid-radical inhibitors changed the outcome.\nIn human H358 and A549 cells, deferoxamine, ferrostatin-1 and liproxstatin-1 reversed cucurbitacin B growth inhibition, supporting a ferroptotic component.\nModel: Human NSCLC cell experiments.\nLimitations: Rescue does not establish exclusive cell-death causation or clinical cancer efficacy.\nEvidence access: Primary abstract\nCucurbitacin B targets STAT3 to induce ferroptosis in non-small cell lung cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38950838/ · DOI 10.1016/j.ejphar.2024.176805","model_system":"Human NSCLC cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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