{"id":"fb0d0367-166a-5766-bf02-a36dde66ca19","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-mtrr-mouse-ratio","predicate":"impairment_has_tissue_specific_effect_on","statement":"Mtrr gene-trap mice did not show decreased SAM:SAH ratios in most tissues despite disturbed remethylation.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"08a05d7b-21b6-552b-968f-d3b62ea5938d","mechanism_event_label":"The ratio did not fall uniformly.","subject":{"id":"db2f9c35-40d4-5c0d-a225-d9d292b717f6","slug":"mtrr-mouse-gene","display_name":"Mtrr gene (Mus musculus)","entity_type_key":"gene"},"object":{"id":"78df8eff-d4a7-5fbb-99b4-3de6fef8dd0f","slug":"sam-sah-ratio","display_name":"SAM:SAH concentration ratio","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"08a05d7b-21b6-552b-968f-d3b62ea5938d","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-mtrr-mouse-ratio-event","event_type":"observed_intervention","label":"The ratio did not fall uniformly.","description":"Mtrr gene-trap mice did not show decreased SAM:SAH ratios in most tissues despite disturbed remethylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"numerator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e84829b1-0607-558e-8add-aaa3af4747e7","slug":"s-adenosylhomocysteine","display_name":"S-Adenosyl-L-homocysteine","entity_type_key":"small_molecule"},"role":"denominator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"db2f9c35-40d4-5c0d-a225-d9d292b717f6","slug":"mtrr-mouse-gene","display_name":"Mtrr gene (Mus musculus)","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"78df8eff-d4a7-5fbb-99b4-3de6fef8dd0f","slug":"sam-sah-ratio","display_name":"SAM:SAH concentration ratio","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Mtrr gene-trap hypomorphic mice and controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ratio is not DNA methylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The ratio did not fall uniformly.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[elmore-2007] Metabolic derangement of methionine and folate metabolism in mice deficient in methionine synthase reductase (2007). https://pubmed.ncbi.nlm.nih.gov/17369066/ DOI: 10.1016/j.ymgme.2007.02.001","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Assayed tissue panel","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4b236739-fb32-5291-bef4-157499a925ab","evidence_kind":"source_excerpt","locator":"Lines 588-597","start_line":588,"end_line":597,"excerpt":"### folate-methyl-mtrr-mouse-ratio\nMtrr gene-trap mice did not show decreased SAM:SAH ratios in most tissues despite disturbed remethylation.\nCondition category: machinery_impairment\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The ratio did not fall uniformly.\norganism: Mus musculus\ntissue_or_cell_type: Assayed tissue panel\nexperimental_model: Mtrr gene-trap hypomorphic mice and controls.\nlimitations: Ratio is not DNA methylation.\n[elmore-2007] Metabolic derangement of methionine and folate metabolism in mice deficient in methionine synthase reductase (2007). https://pubmed.ncbi.nlm.nih.gov/17369066/ DOI: 10.1016/j.ymgme.2007.02.001","model_system":"Mtrr gene-trap hypomorphic mice and controls.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [elmore-2007] Metabolic derangement of methionine and folate metabolism in mice deficient in methionine synthase reductase (2007). https://pubmed.ncbi.nlm.nih.gov/17369066/ DOI: 10.1016/j.ymgme.2007.02.001","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}