{"id":"fb02e62a-172d-594a-9578-6b29b960c2cb","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-parp-loss","predicate":"silencing_blocks","statement":"Silencing PARP1 abolished the one-hour NAD increase induced by low-dose resveratrol in human HeLa cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"215d9410-771a-549d-a7f5-78bb932072ba","mechanism_event_label":"Removing a signaling component prevented the rebound.","subject":{"id":"d5ab002d-61f0-502f-951a-00b96f059988","slug":"parp1","display_name":"Human PARP1","entity_type_key":"protein"},"object":{"id":"07ca7f07-7da8-5fb0-bb3e-11ebca34ccd1","slug":"human-hela-nad-content","display_name":"NAD content in human HeLa cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"215d9410-771a-549d-a7f5-78bb932072ba","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-parp-loss-event","event_type":"observed_relationship","label":"Removing a signaling component prevented the rebound.","description":"Silencing PARP1 abolished the one-hour NAD increase induced by low-dose resveratrol in human HeLa cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d5ab002d-61f0-502f-951a-00b96f059988","slug":"parp1","display_name":"Human PARP1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"07ca7f07-7da8-5fb0-bb3e-11ebca34ccd1","slug":"human-hela-nad-content","display_name":"NAD content in human HeLa cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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