{"id":"fae8e3b7-857b-56aa-9fc0-a3bc11d36184","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-gpr142-sensing","predicate":"activates","statement":"Phenylalanine activated transfected human and mouse GPR142 IP1 signaling with EC50 values around 3.5 and 2.8 mM, respectively.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7da29541-fb9b-5d84-85b8-18841bf35454","mechanism_event_label":"A second amino-acid receptor can respond to phenylalanine.","subject":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"object":{"id":"104c7575-b998-58cc-846f-61f1628079a6","slug":"gpr142","display_name":"Human GPR142","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7da29541-fb9b-5d84-85b8-18841bf35454","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-gpr142-sensing-event","event_type":"observed_relationship","label":"A second amino-acid receptor can respond to phenylalanine.","description":"Phenylalanine activated transfected human and mouse GPR142 IP1 signaling with EC50 values around 3.5 and 2.8 mM, respectively.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"104c7575-b998-58cc-846f-61f1628079a6","slug":"gpr142","display_name":"Human GPR142","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a1317efc-1f28-5b59-be4d-1f9b051120af","slug":"mouse-gpr142","display_name":"Mouse Gpr142","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human or mouse receptor expressed in HEK293 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar cell-assay potency does not prove that GPR142 mediates every whole-animal phenylalanine response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second amino-acid receptor can respond to phenylalanine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"GPR142 Controls Tryptophan-Induced Insulin and Incretin Hormone Secretion to Improve Glucose Metabolism. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27322810/ · DOI 10.1371/journal.pone.0157298","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9210f5f5-b919-54a3-8eaf-0b2d9288a459","evidence_kind":"source_excerpt","locator":"Lines 294-300","start_line":294,"end_line":300,"excerpt":"## l-phenylalanine-gpr142-sensing\nA second amino-acid receptor can respond to phenylalanine.\nPhenylalanine activated transfected human and mouse GPR142 IP1 signaling with EC50 values around 3.5 and 2.8 mM, respectively.\nModel: Human or mouse receptor expressed in HEK293 cells.\nLimitations: Millimolar cell-assay potency does not prove that GPR142 mediates every whole-animal phenylalanine response.\nEvidence access: Primary full text\nGPR142 Controls Tryptophan-Induced Insulin and Incretin Hormone Secretion to Improve Glucose Metabolism. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27322810/ · DOI 10.1371/journal.pone.0157298","model_system":"Human or mouse receptor expressed in HEK293 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4d53adcd4acc7a9aecf44ccb2623ec540c9d221a0f7c00002585870b39b8ffb8","revision_id":"fd182a81-4ab7-559a-9c77-fb4cf72982ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}