{"id":"faa36ea1-49aa-5bf8-918c-97b8bd4481c5","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-ninj1-human-clustering","predicate":"inhibits_tested","statement":"In primary human macrophages, 50 mM glycine inhibited pyroptosis-associated NINJ1 clustering and membrane rupture; NINJ1 knockdown produced a similar rupture phenotype.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"71f5de21-4e8a-5fcc-a9e1-bc0bc2638ecd","mechanism_event_label":"The membrane effect also occurred in human cells at a much higher experimental exposure.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"0784250d-c308-508d-8805-a0ed777463d5","slug":"ninj1","display_name":"Human ninjurin-1 / NINJ1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"71f5de21-4e8a-5fcc-a9e1-bc0bc2638ecd","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-ninj1-human-clustering-event","event_type":"observed_relationship","label":"The membrane effect also occurred in human cells at a much higher experimental exposure.","description":"In primary human macrophages, 50 mM glycine inhibited pyroptosis-associated NINJ1 clustering and membrane rupture; NINJ1 knockdown produced a similar rupture phenotype.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0784250d-c308-508d-8805-a0ed777463d5","slug":"ninj1","display_name":"Human ninjurin-1 / NINJ1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1b850407-e122-55ce-937e-fe4197dbaaa7","slug":"human-macrophage-plasma-membrane-rupture","display_name":"Plasma membrane rupture in human macrophages","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human monocyte-derived macrophages, LPS/nigericin, native-protein analysis and knockdown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direct or indirect target engagement remains unresolved; 50 mM bath exposure is not a dietary plasma concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The membrane effect also occurred in human cells at a much higher experimental exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36468682/ · DOI 10.7554/eLife.78609","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bf99b4cf-899e-540b-81ba-75b50f6fbccc","evidence_kind":"source_excerpt","locator":"Lines 362-368","start_line":362,"end_line":368,"excerpt":"## glycine-ninj1-human-clustering\nThe membrane effect also occurred in human cells at a much higher experimental exposure.\nIn primary human macrophages, 50 mM glycine inhibited pyroptosis-associated NINJ1 clustering and membrane rupture; NINJ1 knockdown produced a similar rupture phenotype.\nModel: Human monocyte-derived macrophages, LPS/nigericin, native-protein analysis and knockdown.\nLimitations: Direct or indirect target engagement remains unresolved; 50 mM bath exposure is not a dietary plasma concentration.\nEvidence access: Primary full text\nGlycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36468682/ · DOI 10.7554/eLife.78609","model_system":"Human monocyte-derived macrophages, LPS/nigericin, native-protein analysis and knockdown.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}