{"id":"fa7ddbbd-8628-5785-ad44-cc3a00b144e9","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-isoprostane-catabolism","predicate":"inhibits","statement":"Adding CLA inhibited conversion of 8-iso-PGF2alpha to its dinor metabolite in normal and adrenoleukodystrophy fibroblasts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e628e660-a6e3-558c-994a-8941b9197f1a","mechanism_event_label":"A higher oxidation marker can reflect slower removal.","subject":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"object":{"id":"787e5fbb-46d6-594b-8c09-61ce5c0bb931","slug":"human-fibroblast-isoprostane-catabolism","display_name":"Human fibroblast isoprostane conversion to dinor metabolite","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e628e660-a6e3-558c-994a-8941b9197f1a","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-isoprostane-catabolism-event","event_type":"observed_relationship","label":"A higher oxidation marker can reflect slower removal.","description":"Adding CLA inhibited conversion of 8-iso-PGF2alpha to its dinor metabolite in normal and adrenoleukodystrophy fibroblasts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"787e5fbb-46d6-594b-8c09-61ce5c0bb931","slug":"human-fibroblast-isoprostane-catabolism","display_name":"Human fibroblast isoprostane conversion to dinor metabolite","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"03e13409-d8c4-540e-a186-eeed0d71b5c1","slug":"8-iso-pgf2alpha","display_name":"8-iso-Prostaglandin F2alpha","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b628f5e2-b123-58aa-9f05-51465f093007","slug":"2-3-dinor-8-iso-pgf2alpha","display_name":"2,3-Dinor-8-iso-prostaglandin F2alpha","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human fibroblasts; accompanying rat experiments showed reduced dinor products.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not prove all CLA-associated isoprostane increases are harmless or unrelated to oxidation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A higher oxidation marker can reflect slower removal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Impairment of 8-iso-PGF(2ALPHA) isoprostane metabolism by dietary conjugated linoleic acid (CLA). · 2009 · https://pubmed.ncbi.nlm.nih.gov/19403295/ · DOI 10.1016/j.plefa.2009.02.008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a6b8ed0b-4053-51d4-be77-19dca81f4520","evidence_kind":"source_excerpt","locator":"Lines 358-364","start_line":358,"end_line":364,"excerpt":"## cla-isoprostane-catabolism\nA higher oxidation marker can reflect slower removal.\nAdding CLA inhibited conversion of 8-iso-PGF2alpha to its dinor metabolite in normal and adrenoleukodystrophy fibroblasts.\nModel: Human fibroblasts; accompanying rat experiments showed reduced dinor products.\nLimitations: Does not prove all CLA-associated isoprostane increases are harmless or unrelated to oxidation.\nEvidence access: Primary abstract\nImpairment of 8-iso-PGF(2ALPHA) isoprostane metabolism by dietary conjugated linoleic acid (CLA). · 2009 · https://pubmed.ncbi.nlm.nih.gov/19403295/ · DOI 10.1016/j.plefa.2009.02.008","model_system":"Human fibroblasts; accompanying rat experiments showed reduced dinor products.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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