{"id":"fa35ab21-af84-5c38-a44c-99158db98be7","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:human-pcc-biotin","predicate":"contains-cofactor","statement":"PCC partially purified from control human fibroblasts contained biotin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7922749d-4aa6-58ba-94a6-72f2e856e8fb","mechanism_event_label":"Human fibroblast PCC carried biotin, a different vitamin required upstream of the B12-dependent step.","subject":{"id":"54eb0f2f-091c-54cd-b4a1-a528b994560a","slug":"human-propionyl-coa-carboxylase","display_name":"Human propionyl-CoA carboxylase / PCC","entity_type_key":"protein_complex"},"object":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"7922749d-4aa6-58ba-94a6-72f2e856e8fb","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:human-pcc-biotin-event","event_type":"biochemical_relationship","label":"Human fibroblast PCC carried biotin, a different vitamin required upstream of the B12-dependent step.","description":"PCC partially purified from control human fibroblasts contained biotin.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"54eb0f2f-091c-54cd-b4a1-a528b994560a","slug":"human-propionyl-coa-carboxylase","display_name":"Human propionyl-CoA carboxylase / PCC","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Indexed primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Partially purified human fibroblast PCC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Control fibroblast enzyme fraction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biotin content in an enzyme preparation; does not establish whole-body biotin thresholds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human fibroblast PCC carried biotin, a different vitamin required upstream of the B12-dependent step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hsia-1979-pcc] Human propionyl CoA carboxylase: some properties of the partially purified enzyme in fibroblasts from controls and patients with propionic acidemia. (1979). https://pubmed.ncbi.nlm.nih.gov/481943/ DOI: 10.1203/00006450-197906000-00005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Fibroblasts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"35ed5cd2-e415-581a-9c9b-49a0e43bd3da","evidence_kind":"source_excerpt","locator":"Lines 1145-1157","start_line":1145,"end_line":1157,"excerpt":"### human-pcc-biotin\nPCC partially purified from control human fibroblasts contained biotin.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Human fibroblast PCC carried biotin, a different vitamin required upstream of the B12-dependent step.\norganism: Homo sapiens\ntissue_or_cell_type: Fibroblasts\nexperimental_model: Partially purified human fibroblast PCC\nlimitations: Biotin content in an enzyme preparation; does not establish whole-body biotin thresholds.\nexposure: Control fibroblast enzyme fraction\ncross_nutrient: true\nevidence_location: Indexed primary abstract\n[hsia-1979-pcc] Human propionyl CoA carboxylase: some properties of the partially purified enzyme in fibroblasts from controls and patients with propionic acidemia. (1979). https://pubmed.ncbi.nlm.nih.gov/481943/ DOI: 10.1203/00006450-197906000-00005","model_system":"Partially purified human fibroblast PCC","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hsia-1979-pcc] Human propionyl CoA carboxylase: some properties of the partially purified enzyme in fibroblasts from controls and patients with propionic acidemia. (1979). https://pubmed.ncbi.nlm.nih.gov/481943/ DOI: 10.1203/00006450-197906000-00005","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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