{"id":"f9e54f6a-cf10-5662-a5ba-5177b8c0f5f2","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-gss-magnesium","predicate":"coordinates_in","statement":"Human GSS crystallized with two magnesium ions, ADP, glutathione and sulfate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4e9f06e3-b2cd-5ff6-af40-78a3d4c81ca1","mechanism_event_label":"The synthesis enzyme has a metal-containing nucleotide-binding environment.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"4f530f0d-22df-58fb-82a8-976e76f875cb","slug":"gss","display_name":"Human glutathione synthetase / GSS","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4e9f06e3-b2cd-5ff6-af40-78a3d4c81ca1","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-gss-magnesium-event","event_type":"biochemical_relationship","label":"The synthesis enzyme has a metal-containing nucleotide-binding environment.","description":"Human GSS crystallized with two magnesium ions, ADP, glutathione and sulfate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"reaction_energy_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4f530f0d-22df-58fb-82a8-976e76f875cb","slug":"gss","display_name":"Human glutathione synthetase / GSS","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glutathione-research/10369661.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ccdd02e451ba6a6d432012c382911ff7fac556e99f9e8d60ce9f3dc94baf9941\", \"start_char\": 0, \"end_char\": 1327, \"text_sha256\": \"ccdd02e451ba6a6d432012c382911ff7fac556e99f9e8d60ce9f3dc94baf9941\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystal structure and disease-variant mapping","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ADP/GSH/sulfate-bound structure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Two Mg ions observed; structure is not a dietary-magnesium depletion experiment. The main synthesis reaction is reused from its existing claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glutathione research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The synthesis enzyme has a metal-containing nucleotide-binding environment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[glutathione-p10369661] Molecular basis of glutathione synthetase deficiency and a rare gene permutation event. (1999). https://pubmed.ncbi.nlm.nih.gov/10369661/ DOI: 10.1093/emboj/18.12.3204","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified GSS","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8a3cd0fd-f543-567b-99c5-16a2f339479c","evidence_kind":"source_excerpt","locator":"Lines 333-344","start_line":333,"end_line":344,"excerpt":"### glutathione-gss-magnesium\nHuman GSS crystallized with two magnesium ions, ADP, glutathione and sulfate.\nCondition category: normal\nnutrient_topic: Glutathione research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The synthesis enzyme has a metal-containing nucleotide-binding environment.\norganism: Human enzyme\ntissue_or_cell_type: Purified GSS\nexperimental_model: Crystal structure and disease-variant mapping\nlimitations: Two Mg ions observed; structure is not a dietary-magnesium depletion experiment. The main synthesis reaction is reused from its existing claim.\nexposure: ADP/GSH/sulfate-bound structure\nevidence_span: {\"source_cache\": \"artifacts/glutathione-research/10369661.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ccdd02e451ba6a6d432012c382911ff7fac556e99f9e8d60ce9f3dc94baf9941\", \"start_char\": 0, \"end_char\": 1327, \"text_sha256\": \"ccdd02e451ba6a6d432012c382911ff7fac556e99f9e8d60ce9f3dc94baf9941\"}\n[glutathione-p10369661] Molecular basis of glutathione synthetase deficiency and a rare gene permutation event. (1999). https://pubmed.ncbi.nlm.nih.gov/10369661/ DOI: 10.1093/emboj/18.12.3204","model_system":"Crystal structure and disease-variant mapping","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [glutathione-p10369661] Molecular basis of glutathione synthetase deficiency and a rare gene permutation event. (1999). https://pubmed.ncbi.nlm.nih.gov/10369661/ DOI: 10.1093/emboj/18.12.3204","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9b7417fd-059d-5683-b452-71d1e3cf82d0","stable_key":"import-db0fc92e-b5ef-5667-a4c5-3ef257edbc9b","title":"Glutathione: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2a5740c4a7e770aafe883fea47909b18bf5e174f211749935130a50f9dea2753","revision_id":"a64016dd-4b72-5954-a72d-0e61de854073","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}