{"id":"f9ccf0bb-2313-5b09-aff3-8ae31bdafc5e","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-mitochondrial-gc2","predicate":"transports","statement":"Reconstituted human glutamate carrier 2 also supported glutamate/proton-coupled transport, with kinetic and expression differences from carrier 1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f61504bc-0c9d-5cb9-9e5d-d867d1256386","mechanism_event_label":"A related carrier is a separate node with its own properties.","subject":{"id":"6ded93b3-f9a9-519f-9cfe-aee52c814616","slug":"slc25a18","display_name":"Human mitochondrial glutamate carrier 2 / SLC25A18","entity_type_key":"protein"},"object":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f61504bc-0c9d-5cb9-9e5d-d867d1256386","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-mitochondrial-gc2-event","event_type":"observed_relationship","label":"A related carrier is a separate node with its own properties.","description":"Reconstituted human glutamate carrier 2 also supported glutamate/proton-coupled transport, with kinetic and expression differences from carrier 1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6ded93b3-f9a9-519f-9cfe-aee52c814616","slug":"slc25a18","display_name":"Human mitochondrial glutamate carrier 2 / SLC25A18","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b5c995ba-9aa1-5aad-ae2a-11c0c22f68f7","slug":"slc25a22","display_name":"Human mitochondrial glutamate carrier 1 / SLC25A22","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human SLC25A18 compared with SLC25A22 in vesicle transport assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The proposed allocation to basal versus high-demand metabolism was an interpretation, not a universal tissue ranking.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A related carrier is a separate node with its own properties.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification of the mitochondrial glutamate transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11897791/ · DOI 10.1074/jbc.M201572200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"The record names the proton coupling and not which way glutamate crossed the membrane.","entity":null},{"dimension":"transport_pool","value_text":"the mitochondrial matrix","comparator":null,"unit":null,"notes":"The record names the proton coupling and not which way glutamate crossed the membrane.","entity":null}],"evidence":[{"id":"dd68bbd8-28a4-5d83-bc89-c9d0a6d45194","evidence_kind":"source_excerpt","locator":"Lines 90-96","start_line":90,"end_line":96,"excerpt":"## glutamate-mitochondrial-gc2\nA related carrier is a separate node with its own properties.\nReconstituted human glutamate carrier 2 also supported glutamate/proton-coupled transport, with kinetic and expression differences from carrier 1.\nModel: Recombinant human SLC25A18 compared with SLC25A22 in vesicle transport assays.\nLimitations: The proposed allocation to basal versus high-demand metabolism was an interpretation, not a universal tissue ranking.\nEvidence access: Primary abstract\nIdentification of the mitochondrial glutamate transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11897791/ · DOI 10.1074/jbc.M201572200","model_system":"Recombinant human SLC25A18 compared with SLC25A22 in vesicle transport assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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