{"id":"f9c03ee9-9c42-5b58-a11d-5547f3815166","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:trpm6-mg-entry","predicate":"supports","statement":"Recombinant TRPM6 expression generated Mg2+-permeable currents, supporting its participation in apical epithelial Mg entry.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9fafeea0-396c-5796-b6b8-fdc4afb51bde","mechanism_event_label":"TRPM6 is part of the route by which magnesium crosses an epithelial cell membrane.","subject":{"id":"877a2fb0-067c-51ca-afea-f63ff16affc8","slug":"trpm6","display_name":"TRPM6","entity_type_key":"protein"},"object":{"id":"2c31af15-4eba-5fdc-9a37-11104bd19c82","slug":"cellular-magnesium-influx","display_name":"Cellular magnesium influx","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9fafeea0-396c-5796-b6b8-fdc4afb51bde","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:trpm6-mg-entry-event","event_type":"biochemical_relationship","label":"TRPM6 is part of the route by which magnesium crosses an epithelial cell membrane.","description":"Recombinant TRPM6 expression generated Mg2+-permeable currents, supporting its participation in apical epithelial Mg entry.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"transported-ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"also-permeant-ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"16ade5d1-e269-5904-9ab1-b285509b7aef","slug":"trpm7","display_name":"TRPM7","entity_type_key":"protein"},"role":"potential-native-channel-partner","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"877a2fb0-067c-51ca-afea-f63ff16affc8","slug":"trpm6","display_name":"TRPM6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2c31af15-4eba-5fdc-9a37-11104bd19c82","slug":"cellular-magnesium-influx","display_name":"Cellular magnesium influx","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence-system","value_text":"Recombinant channel recordings and tissue localization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant channel recordings and tissue localization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression-system currents do not establish the stoichiometry of native channels; TRPM7 can contribute.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein; mouse tissues","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"TRPM6 is part of the route by which magnesium crosses an epithelial cell membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[voets-2004-trpm6] TRPM6 forms the Mg2+ influx channel involved in intestinal and renal Mg2+ absorption. (2004). https://pubmed.ncbi.nlm.nih.gov/14576148/ DOI: 10.1074/jbc.M311201200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"HEK expression cells; intestine and distal renal tubule","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK expression cells; intestine and distal renal tubule","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_direction","value_text":"Extracellular or luminal compartment toward cytosol.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c53f392b-d24e-56a3-ac85-1d34360afb17","evidence_kind":"source_excerpt","locator":"Lines 869-881","start_line":869,"end_line":881,"excerpt":"### trpm6-mg-entry\nRecombinant TRPM6 expression generated Mg2+-permeable currents, supporting its participation in apical epithelial Mg entry.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: TRPM6 is part of the route by which magnesium crosses an epithelial cell membrane.\norganism: Human protein; mouse tissues\ntissue_or_cell_type: HEK expression cells; intestine and distal renal tubule\nexperimental_model: Recombinant channel recordings and tissue localization\nlimitations: Expression-system currents do not establish the stoichiometry of native channels; TRPM7 can contribute.\ntransport_direction: Extracellular or luminal compartment toward cytosol.\nevidence-system: Recombinant channel recordings and tissue localization\ntissue: HEK expression cells; intestine and distal renal tubule\n[voets-2004-trpm6] TRPM6 forms the Mg2+ influx channel involved in intestinal and renal Mg2+ absorption. 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