{"id":"f9b4b1bd-bfc5-549f-9732-37bd61adf868","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-biofilm-calcium","predicate":"lowers","statement":"Repeated sucrose exposure lowered calcium concentration in whole in-situ dental biofilm relative to water.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a2d476e4-d430-5e78-b33d-b426ad30ef58","mechanism_event_label":"Repeated sucrose exposure lowered calcium concentration in whole in-situ dental biofilm relative to water.","subject":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"object":{"id":"a17cb7ba-e356-5cae-9399-786d51584315","slug":"human-whole-dental-biofilm-calcium","display_name":"Calcium concentration in human in-situ whole dental biofilm","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a2d476e4-d430-5e78-b33d-b426ad30ef58","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-biofilm-calcium-event","event_type":"observed_relationship","label":"Repeated sucrose exposure lowered calcium concentration in whole in-situ dental biofilm relative to water.","description":"Repeated sucrose exposure lowered calcium concentration in whole in-situ dental biofilm relative to water.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a17cb7ba-e356-5cae-9399-786d51584315","slug":"human-whole-dental-biofilm-calcium","display_name":"Calcium concentration in human in-situ whole dental biofilm","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Sucrose or glucose+fructose solution 8 times/day versus water; concentration and sample size unrecovered","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"14 days; 24-hour carbohydrate withdrawal follow-up","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract/metadata; unrecovered methods explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human volunteers wearing enamel blocks in palatal appliances","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Sucrose and local mineral availability","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Local biofilm calcium, phosphate and fluoride pools are not systemic nutrient status. Whole-biofilm and fluid results differ; no dietary calcium-deficiency inference.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sucrose chapter; direct sucrose observations are distinguished from shared component metabolism.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human volunteers wearing enamel blocks in palatal appliances","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Repeated sucrose exposure lowered calcium concentration in whole in-situ dental biofilm relative to water.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ca, Pi, and F in the fluid of biofilm formed under sucrose. (2006). https://pubmed.ncbi.nlm.nih.gov/16931867/ DOI: 10.1177/154405910608500911","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Repeated local oral exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Whole dental biofilm versus its fluid compartment","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ace10e1d-f483-5d94-bb39-68bc027fa354","evidence_kind":"source_excerpt","locator":"Lines 439-449","start_line":439,"end_line":449,"excerpt":"## sucrose-biofilm-calcium\nRepeated sucrose exposure lowered calcium concentration in whole in-situ dental biofilm relative to water.\nModel/species: Human volunteers wearing enamel blocks in palatal appliances\nTissue: Whole dental biofilm versus its fluid compartment\nExposure: Sucrose or glucose+fructose solution 8 times/day versus water; concentration and sample size unrecovered\nRoute: Repeated local oral exposure\nDuration: 14 days; 24-hour carbohydrate withdrawal follow-up\nExposure scope: Sucrose and local mineral availability\nLimits: Local biofilm calcium, phosphate and fluoride pools are not systemic nutrient status. Whole-biofilm and fluid results differ; no dietary calcium-deficiency inference.\nReference: Ca, Pi, and F in the fluid of biofilm formed under sucrose. (2006). https://pubmed.ncbi.nlm.nih.gov/16931867/ DOI: 10.1177/154405910608500911\nAccess: Primary abstract/metadata; unrecovered methods explicitly retained.","model_system":"Human volunteers wearing enamel blocks in palatal appliances","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8e24ddf4-84ee-57b4-9fd8-093547432ece","stable_key":"import-c932089b-ebc9-5f39-b2e5-84af74f9f6d0","title":"Sucrose: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific sucrose curation with shared canonical claims retained by identity. Primary-study citations, negative findings, exposure details and limitations preserved. Not publisher full text.","file_path":"","sha256":"5e68ccbade7a80fc03778495756f0eeeeba5038cd10d5bef8e33cb1e93e4b044","revision_id":"6edb8ffc-11c5-5cd9-852c-85adb71165a8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}