{"id":"f9ae6d0b-7ce9-5462-baf0-fd6e7efbd15d","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-apo-p21-p27","predicate":"increased","statement":"The metabolite increased p21 and p27 protein in the tested bronchial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d3583ec6-ec5a-50d4-a11c-7f0e41229384","mechanism_event_label":"Proteins that restrain cell-cycle progression increased.","subject":{"id":"09d5d992-0670-5714-9cfc-992c18bbc0dd","slug":"apo-10-lycopenoic-acid","display_name":"Apo-10-prime-lycopenoic acid","entity_type_key":"small_molecule"},"object":{"id":"f1e247dc-b0f7-5a28-9e85-d189fecd10c0","slug":"bronchial-p21-p27-abundance","display_name":"p21 and p27 abundance in experimental bronchial cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d3583ec6-ec5a-50d4-a11c-7f0e41229384","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-apo-p21-p27-event","event_type":"biochemical_relationship","label":"Proteins that restrain cell-cycle progression increased.","description":"The metabolite increased p21 and p27 protein in the tested bronchial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"09d5d992-0670-5714-9cfc-992c18bbc0dd","slug":"apo-10-lycopenoic-acid","display_name":"Apo-10-prime-lycopenoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f1e247dc-b0f7-5a28-9e85-d189fecd10c0","slug":"bronchial-p21-p27-abundance","display_name":"p21 and p27 abundance in experimental bronchial cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/17420169.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84a4b4088d833621e68106129dcbe8ddda9e743ec613cebdd4ce9cf03daeb15c\", \"start_char\": 0, \"end_char\": 1675, \"text_sha256\": \"84a4b4088d833621e68106129dcbe8ddda9e743ec613cebdd4ce9cf03daeb15c\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-cycle assays and NNK-induced mouse tumor model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Apo-10-prime-lycopenoic acid; mouse diets 10, 40 or 120 mg/kg","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Metabolite exposure, not a clinical lycopene trial; tumor multiplicity is not human survival.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human bronchial cell lines; A/J mice separately specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Proteins that restrain cell-cycle progression increased.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p17420169] Apo-10'-lycopenoic acid inhibits lung cancer cell growth in vitro, and suppresses lung tumorigenesis in the A/J mouse model in vivo. (2007). https://pubmed.ncbi.nlm.nih.gov/17420169/ DOI: 10.1093/carcin/bgm076","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Bronchial epithelium and lung tumors","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f07f30b4-c1d8-542c-9979-dcbdc08b66df","evidence_kind":"source_excerpt","locator":"Lines 728-739","start_line":728,"end_line":739,"excerpt":"### lycopene-apo-p21-p27\nThe metabolite increased p21 and p27 protein in the tested bronchial cells.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Proteins that restrain cell-cycle progression increased.\norganism: Human bronchial cell lines; A/J mice separately specified\ntissue_or_cell_type: Bronchial epithelium and lung tumors\nexperimental_model: Cell-cycle assays and NNK-induced mouse tumor model\nlimitations: Metabolite exposure, not a clinical lycopene trial; tumor multiplicity is not human survival.\nexposure: Apo-10-prime-lycopenoic acid; mouse diets 10, 40 or 120 mg/kg\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/17420169.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84a4b4088d833621e68106129dcbe8ddda9e743ec613cebdd4ce9cf03daeb15c\", \"start_char\": 0, \"end_char\": 1675, \"text_sha256\": \"84a4b4088d833621e68106129dcbe8ddda9e743ec613cebdd4ce9cf03daeb15c\"}\n[lycopene-p17420169] Apo-10'-lycopenoic acid inhibits lung cancer cell growth in vitro, and suppresses lung tumorigenesis in the A/J mouse model in vivo. 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