{"id":"f9aca51e-22e8-5584-9d11-909f91414847","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:yeast-redox-loss-yeast-yap1","predicate":"decreases_in_recorded_experiment","statement":"Loss of yeast-yap1 increased susceptibility in the allicin arm of the yeast chemical-genetic comparison.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"631d5a84-957c-5f8a-adca-7f77fac2d3a7","mechanism_event_label":"Loss of yeast-yap1 increased susceptibility in the allicin arm of the yeast chemical-genetic comparison.","subject":{"id":"320766bd-6908-5f99-96b7-787830529fa4","slug":"yeast-yap1","display_name":"Saccharomyces cerevisiae oxidative-stress transcription factor / Yap1","entity_type_key":"protein"},"object":{"id":"29ccc26a-b3de-57b2-b46d-5f6206054867","slug":"yeast-allicin-growth","display_name":"Saccharomyces cerevisiae growth during allicin exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"631d5a84-957c-5f8a-adca-7f77fac2d3a7","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:yeast-redox-loss-yeast-yap1-event","event_type":"experimental_observation","label":"Loss of yeast-yap1 increased susceptibility in the allicin arm of the yeast chemical-genetic comparison.","description":"**Redox-defense impairment.** A study comparing allicin with a sulfilimine analogue included allicin-exposed Saccharomyces cerevisiae mutants. The GLR1, ZWF1, and YAP1 perturbations connect susceptibility to glutathione recycling, NADPH supply, and stress regulation; effects depended on concentration and compound. The analogue was more potent in some low-concentration comparisons, and its results must not be copied onto allicin. TRX2 loss had a much smaller effect in this system. Human B2 deficiency, G6PD deficiency, or selenium status cannot be assigned the yeast phenotype without direct evidence. [Horn 2020](https://pmc.ncbi.nlm.nih.gov/articles/PMC7694261/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"320766bd-6908-5f99-96b7-787830529fa4","slug":"yeast-yap1","display_name":"Saccharomyces cerevisiae oxidative-stress transcription factor / Yap1","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"yeast-yap1 deletion with allicin","sequence_order":0,"notes":""},{"entity":{"id":"29ccc26a-b3de-57b2-b46d-5f6206054867","slug":"yeast-allicin-growth","display_name":"Saccharomyces cerevisiae growth during allicin exposure","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"test exposure","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"redox-network requirement, not directly depleted in this contrast","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"thiol-network context","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"present","comparator":"Wild-type yeast at the same allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"deleted","comparator":"Wild-type yeast at the same allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"yeast-yap1","display_name":"Saccharomyces cerevisiae oxidative-stress transcription factor / Yap1","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"yeast-yap1 deletion with allicin\", \"comparator\": \"Wild-type yeast at the same allicin exposure\", \"endpoint\": \"Loss of yeast-yap1 increased susceptibility in the allicin arm of the yeast chemical-genetic comparison.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"yeast-yap1\", \"state\": \"deleted\"}, {\"entity_slug\": \"allicin\", \"state\": \"present\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Saccharomyces cerevisiae; separate gene deletions and allicin exposures compared with an analogue; Figure 3.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Keep dose and compound distinctions: analogue effects cannot substitute for allicin. Not a human nutrient-deficiency phenotype.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of yeast-yap1 increased susceptibility in the allicin arm of the yeast chemical-genetic comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Sulfilimine Analogue of Allicin, <i>S</i>-Allyl-<i>S</i>-(<i>S</i>-allyl)-<i>N</i>-Cyanosulfilimine, Is Antimicrobial and Reacts with Glutathione. | 2020 | DOI 10.3390/antiox9111086 | PMID 33158268 | https://pubmed.ncbi.nlm.nih.gov/33158268/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC7694261/ | https://doi.org/10.3390/antiox9111086","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 49-49; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"8b0eeb06-776e-51f3-819f-78333fbfd19e","evidence_kind":"source_excerpt","locator":"Lines 49-49","start_line":49,"end_line":49,"excerpt":"**Redox-defense impairment.** A study comparing allicin with a sulfilimine analogue included allicin-exposed Saccharomyces cerevisiae mutants. The GLR1, ZWF1, and YAP1 perturbations connect susceptibility to glutathione recycling, NADPH supply, and stress regulation; effects depended on concentration and compound. The analogue was more potent in some low-concentration comparisons, and its results must not be copied onto allicin. TRX2 loss had a much smaller effect in this system. Human B2 deficiency, G6PD deficiency, or selenium status cannot be assigned the yeast phenotype without direct evidence. [Horn 2020](https://pmc.ncbi.nlm.nih.gov/articles/PMC7694261/)","model_system":"Saccharomyces cerevisiae; separate gene deletions and allicin exposures compared with an analogue; Figure 3.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}