{"id":"f9a11122-0a2a-5dc1-8b02-c045976262c8","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-slc25a42-exchange","predicate":"transports_by_counterexchange","statement":"Reconstituted human SLC25A42 transported CoA by counter-exchange and targeted mitochondria, supporting a route for mitochondrial CoA import.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7312614b-8d23-5f6d-a1ee-bef58f118cea","mechanism_event_label":"Cells need transport machinery to supply the mitochondrial CoA compartment.","subject":{"id":"14124a14-1eb8-5e7b-89d2-ad6ed3409238","slug":"slc25a42","display_name":"Human mitochondrial coenzyme A transporter SLC25A42","entity_type_key":"protein"},"object":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"7312614b-8d23-5f6d-a1ee-bef58f118cea","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-slc25a42-exchange-event","event_type":"biochemical_relationship","label":"Cells need transport machinery to supply the mitochondrial CoA compartment.","description":"Reconstituted human SLC25A42 transported CoA by counter-exchange and targeted mitochondria, supporting a route for mitochondrial CoA import.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"136c3764-1b93-5f79-8673-9001cab9bc3d","slug":"adp","display_name":"Adenosine diphosphate","entity_type_key":"small_molecule"},"role":"exchange_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3e44a0fb-9c4e-58b5-95ad-911592c3bb9f","slug":"adenosine-3-5-bisphosphate","display_name":"Adenosine 3′,5′-bisphosphate","entity_type_key":"small_molecule"},"role":"exchange_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9bc4f783-5bbd-51de-b3b1-15b6fa910121","slug":"dephospho-coa","display_name":"3′-Dephospho-coenzyme A","entity_type_key":"small_molecule"},"role":"additional_transported_substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"14124a14-1eb8-5e7b-89d2-ad6ed3409238","slug":"slc25a42","display_name":"Human mitochondrial coenzyme A transporter SLC25A42","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SLC25A42 expressed in E. coli, purified and reconstituted into phospholipid vesicles, plus mitochondrial targeting experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified transporter proteoliposomes; substrate concentrations not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The experiment established exchange, not uniport. The proposed physiological import direction depends on metabolite gradients; the study does not establish that SLC25A42 is the sole importer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cells need transport machinery to supply the mitochondrial CoA compartment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-bio-slc25a42] A novel member of solute carrier family 25 (SLC25A42) is a transporter of coenzyme A and adenosine 3',5'-diphosphate in human mitochondria. (2009). https://pubmed.ncbi.nlm.nih.gov/19429682/ DOI: 10.1074/jbc.m109.014118","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Reconstituted phospholipid vesicles and mitochondrial-targeting experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Counter-exchange transport that the record describes as a route for mitochondrial CoA import.","entity":null},{"dimension":"transport_pool","value_text":"the mitochondrial matrix","comparator":null,"unit":null,"notes":"Counter-exchange transport that the record describes as a route for mitochondrial CoA import.","entity":null}],"evidence":[{"id":"5198688a-9587-5a58-ac7e-38fff4cece22","evidence_kind":"source_excerpt","locator":"Lines 704-715","start_line":704,"end_line":715,"excerpt":"### b5-bio-slc25a42-exchange\nReconstituted human SLC25A42 transported CoA by counter-exchange and targeted mitochondria, supporting a route for mitochondrial CoA import.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Cells need transport machinery to supply the mitochondrial CoA compartment.\norganism: Homo sapiens\ntissue_or_cell_type: Reconstituted phospholipid vesicles and mitochondrial-targeting experiments\nexperimental_model: Human SLC25A42 expressed in E. coli, purified and reconstituted into phospholipid vesicles, plus mitochondrial targeting experiments\nlimitations: The experiment established exchange, not uniport. The proposed physiological import direction depends on metabolite gradients; the study does not establish that SLC25A42 is the sole importer.\nexposure: Purified transporter proteoliposomes; substrate concentrations not extracted.\ncross_nutrient: false\n[b5-bio-slc25a42] A novel member of solute carrier family 25 (SLC25A42) is a transporter of coenzyme A and adenosine 3',5'-diphosphate in human mitochondria. (2009). https://pubmed.ncbi.nlm.nih.gov/19429682/ DOI: 10.1074/jbc.m109.014118","model_system":"Human SLC25A42 expressed in E. coli, purified and reconstituted into phospholipid vesicles, plus mitochondrial targeting experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-bio-slc25a42] A novel member of solute carrier family 25 (SLC25A42) is a transporter of coenzyme A and adenosine 3',5'-diphosphate in human mitochondria. (2009). https://pubmed.ncbi.nlm.nih.gov/19429682/ DOI: 10.1074/jbc.m109.014118","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}