{"id":"f953400c-cf4e-574e-92f2-8a45e7edbc58","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-dopamine-sulfation","predicate":"inhibits","statement":"Tartrazine inhibited dopamine sulfation in human liver cytosol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f8607937-9861-5258-8575-5cb530d2bba8","mechanism_event_label":"Tartrazine inhibited dopamine sulfation in human liver cytosol.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"a04fd7f0-68da-555d-8e60-cd49299b1761","slug":"human-liver-dopamine-sulfation","display_name":"Dopamine sulfation by human liver cytosol","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f8607937-9861-5258-8575-5cb530d2bba8","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-dopamine-sulfation-event","event_type":"observed_relationship","label":"Tartrazine inhibited dopamine sulfation in human liver cytosol.","description":"Tartrazine inhibited dopamine sulfation in human liver cytosol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a04fd7f0-68da-555d-8e60-cd49299b1761","slug":"human-liver-dopamine-sulfation","display_name":"Dopamine sulfation by human liver cytosol","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cca6209-0603-5ab7-96ad-2f3ae4b772d2","slug":"dopamine","display_name":"Dopamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine in an additive-inhibition panel; concentration and substrate amounts not in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Assay interval not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human liver cytosolic preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A functional sulfation assay does not demonstrate direct SULT1A3 binding or altered human brain dopamine. Ethinyloestradiol findings for other additives are not transferred to tartrazine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human liver cytosolic preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine inhibited dopamine sulfation in human liver cytosol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Common food additives are potent inhibitors of human liver 17 alpha-ethinyloestradiol and dopamine sulphotransferases. (1993). https://pubmed.ncbi.nlm.nih.gov/8250957/ DOI: 10.1016/0006-2952(93)90575-h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro cytosolic enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Dopamine sulfation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"032b5595-31db-5bc9-8c86-5024e32c0928","evidence_kind":"source_excerpt","locator":"Lines 259-268","start_line":259,"end_line":268,"excerpt":"## tartrazine-dopamine-sulfation\nTartrazine inhibited dopamine sulfation in human liver cytosol.\nModel/species: Human liver cytosolic preparations\nTissue: Dopamine sulfation\nExposure: Tartrazine in an additive-inhibition panel; concentration and substrate amounts not in abstract\nRoute: In vitro cytosolic enzyme assay\nDuration: Assay interval not specified in accessed abstract\nLimits: A functional sulfation assay does not demonstrate direct SULT1A3 binding or altered human brain dopamine. Ethinyloestradiol findings for other additives are not transferred to tartrazine.\nPrimary reference: Common food additives are potent inhibitors of human liver 17 alpha-ethinyloestradiol and dopamine sulphotransferases. (1993). https://pubmed.ncbi.nlm.nih.gov/8250957/ DOI: 10.1016/0006-2952(93)90575-h\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Human liver cytosolic preparations","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}