{"id":"f91ea23a-b9ef-504a-a8ad-64bc1514a542","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-cyp3a4-activity","predicate":"decreases_activity","statement":"Mangiferin reduced measured CYP3A4 activity in the hepatocyte experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1464a730-a6a9-5639-b008-cec435cce1ea","mechanism_event_label":"A drug-metabolizing enzyme was inhibited under the tested exposure.","subject":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"object":{"id":"54e95ff1-4ffa-5e6f-a4cb-b47e5d2a5bda","slug":"cyp3a4","display_name":"Human cytochrome P450 3A4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"1464a730-a6a9-5639-b008-cec435cce1ea","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-cyp3a4-activity-event","event_type":"biochemical_relationship","label":"A drug-metabolizing enzyme was inhibited under the tested exposure.","description":"Mangiferin reduced measured CYP3A4 activity in the hepatocyte experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"54e95ff1-4ffa-5e6f-a4cb-b47e5d2a5bda","slug":"cyp3a4","display_name":"Human cytochrome P450 3A4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/22815239.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e85ebd0af89f030dbeda45ed922e93a8a616c51f5ce1b64badc44d90791716c0\", \"start_char\": 0, \"end_char\": 1728, \"text_sha256\": \"e85ebd0af89f030dbeda45ed922e93a8a616c51f5ce1b64badc44d90791716c0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human hepatocyte exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mangiferin 50-250 micrograms/mL for 48 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High in vitro concentrations; decreased enzyme activity is not proof of human drug-level changes. Extract and isolated mangiferin are distinct.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A drug-metabolizing enzyme was inhibited under the tested exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p22815239] Mangifera indica L. extract and mangiferin modulate cytochrome P450 and UDP-glucuronosyltransferase enzymes in primary cultures of human hepatocytes. (2013). https://pubmed.ncbi.nlm.nih.gov/22815239/ DOI: 10.1002/ptr.4782","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured hepatocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"30ea8a7f-7477-533a-8f4d-9b612bf23cf7","evidence_kind":"source_excerpt","locator":"Lines 822-833","start_line":822,"end_line":833,"excerpt":"### mangiferin-cyp3a4-activity\nMangiferin reduced measured CYP3A4 activity in the hepatocyte experiment.\nCondition category: normal\nnutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A drug-metabolizing enzyme was inhibited under the tested exposure.\norganism: Homo sapiens\ntissue_or_cell_type: Cultured hepatocytes\nexperimental_model: Primary human hepatocyte exposure\nlimitations: High in vitro concentrations; decreased enzyme activity is not proof of human drug-level changes. Extract and isolated mangiferin are distinct.\nexposure: Mangiferin 50-250 micrograms/mL for 48 hours\nevidence_span: {\"source_cache\": \"artifacts/mangiferin-research/22815239.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e85ebd0af89f030dbeda45ed922e93a8a616c51f5ce1b64badc44d90791716c0\", \"start_char\": 0, \"end_char\": 1728, \"text_sha256\": \"e85ebd0af89f030dbeda45ed922e93a8a616c51f5ce1b64badc44d90791716c0\"}\n[mangiferin-p22815239] Mangifera indica L. extract and mangiferin modulate cytochrome P450 and UDP-glucuronosyltransferase enzymes in primary cultures of human hepatocytes. (2013). https://pubmed.ncbi.nlm.nih.gov/22815239/ DOI: 10.1002/ptr.4782","model_system":"Primary human hepatocyte exposure","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mangiferin-p22815239] Mangifera indica L. extract and mangiferin modulate cytochrome P450 and UDP-glucuronosyltransferase enzymes in primary cultures of human hepatocytes. (2013). https://pubmed.ncbi.nlm.nih.gov/22815239/ DOI: 10.1002/ptr.4782","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"267f942a-695a-5b62-a858-f71c06321ff5","stable_key":"import-475ea55a-65e9-51c3-a539-738a6a8f683f","title":"Mangiferin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"295cb3a13742b3caeed19347ffcbc7863f5372d983da1625a942428919b4dc98","revision_id":"d9c2ee89-ff22-5e86-9d7f-7cb7e17f9ed2","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}