{"id":"f8d88241-0321-55d7-ba5a-9ad10ece6bcc","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cact-exchange","predicate":"exchanges","statement":"Purified rat mitochondrial CACT reconstituted into liposomes exchanged carnitine and transported acylcarnitines of several chain lengths.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e38a3239-c519-5ca3-a5a7-5bb9ec759a48","mechanism_event_label":"A separate carrier moves the loaded molecule across the inner membrane.","subject":{"id":"49164668-8ef7-5bde-afda-1c6aadd87c68","slug":"rat-slc25a20","display_name":"Rat carnitine-acylcarnitine translocase / Slc25a20","entity_type_key":"protein"},"object":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e38a3239-c519-5ca3-a5a7-5bb9ec759a48","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cact-exchange-event","event_type":"observed_relationship","label":"A separate carrier moves the loaded molecule across the inner membrane.","description":"Purified rat mitochondrial CACT reconstituted into liposomes exchanged carnitine and transported acylcarnitines of several chain lengths.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49164668-8ef7-5bde-afda-1c6aadd87c68","slug":"rat-slc25a20","display_name":"Rat carnitine-acylcarnitine translocase / Slc25a20","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f1c881ce-8094-5602-bb97-074377340185","slug":"palmitoylcarnitine","display_name":"Palmitoyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant rat carrier and proteoliposomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport reconstitution is not a human flux measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A separate carrier moves the loaded molecule across the inner membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bacterial overexpression, purification, and reconstitution of the carnitine/acylcarnitine carrier from rat liver mitochondria. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9731180/ · DOI 10.1006/bbrc.1998.9197","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1b48c361-b9e8-5ffa-9d4d-26fdd0a691f2","evidence_kind":"source_excerpt","locator":"Lines 98-104","start_line":98,"end_line":104,"excerpt":"## l-carnitine-cact-exchange\nA separate carrier moves the loaded molecule across the inner membrane.\nPurified rat mitochondrial CACT reconstituted into liposomes exchanged carnitine and transported acylcarnitines of several chain lengths.\nModel: Recombinant rat carrier and proteoliposomes.\nLimitations: Transport reconstitution is not a human flux measurement.\nEvidence access: Primary abstract\nBacterial overexpression, purification, and reconstitution of the carnitine/acylcarnitine carrier from rat liver mitochondria. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9731180/ · DOI 10.1006/bbrc.1998.9197","model_system":"Recombinant rat carrier and proteoliposomes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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