{"id":"f8c8bda0-a293-5198-9498-5b1150132b6a","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-castor-binding","predicate":"binds","statement":"Arginine bound CASTOR1 with an approximate dissociation constant of 30 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bb6a0d99-da1b-5e20-8030-61a90514c1c9","mechanism_event_label":"Arginine is a signal as well as a reaction substrate.","subject":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"object":{"id":"bcea5dc3-78e0-59c6-9d0d-52b90f980fe5","slug":"castor1","display_name":"Human cellular arginine sensor CASTOR1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bb6a0d99-da1b-5e20-8030-61a90514c1c9","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-castor-binding-event","event_type":"observed_relationship","label":"Arginine is a signal as well as a reaction substrate.","description":"Arginine bound CASTOR1 with an approximate dissociation constant of 30 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bcea5dc3-78e0-59c6-9d0d-52b90f980fe5","slug":"castor1","display_name":"Human cellular arginine sensor CASTOR1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mammalian-cell signaling experiments and biochemical CASTOR1 binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding constant is not a human deficiency threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Arginine is a signal as well as a reaction substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26972053/ · DOI 10.1016/j.cell.2016.02.035","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"72721ea7-1c46-5861-a115-b357fbecdc4a","evidence_kind":"source_excerpt","locator":"Lines 174-180","start_line":174,"end_line":180,"excerpt":"## arg-castor-binding\nArginine is a signal as well as a reaction substrate.\nArginine bound CASTOR1 with an approximate dissociation constant of 30 micromolar.\nModel: Mammalian-cell signaling experiments and biochemical CASTOR1 binding.\nLimitations: Binding constant is not a human deficiency threshold.\nEvidence access: Primary abstract\nThe CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26972053/ · DOI 10.1016/j.cell.2016.02.035","model_system":"Mammalian-cell signaling experiments and biochemical CASTOR1 binding.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"ec0e9a3fb72b7485534e4372a074ac7624a399ff962e55690eb462217b5149ec","revision_id":"af027270-5535-51ae-85ff-9c76c518c962","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}