{"id":"f8b195bc-4d4b-58bb-aefe-2ecf4fd7d9f9","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-adma-transport","predicate":"competitively_inhibits","statement":"ADMA produced a competitive inhibition pattern for CAT1-mediated arginine uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ed671264-db30-5e00-95a0-686057d5c9b5","mechanism_event_label":"An endogenous arginine derivative can compete at the entry step.","subject":{"id":"6c8e2535-0002-5b29-bb3d-36cb341b85d2","slug":"asymmetric-dimethylarginine","display_name":"Asymmetric dimethylarginine / ADMA","entity_type_key":"small_molecule"},"object":{"id":"19e0de57-73cc-5896-967b-0423c0f53277","slug":"cat1-arginine-uptake","display_name":"Human CAT1-mediated arginine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ed671264-db30-5e00-95a0-686057d5c9b5","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-adma-transport-event","event_type":"observed_relationship","label":"An endogenous arginine derivative can compete at the entry step.","description":"ADMA produced a competitive inhibition pattern for CAT1-mediated arginine uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6c8e2535-0002-5b29-bb3d-36cb341b85d2","slug":"asymmetric-dimethylarginine","display_name":"Asymmetric dimethylarginine / ADMA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"19e0de57-73cc-5896-967b-0423c0f53277","slug":"cat1-arginine-uptake","display_name":"Human CAT1-mediated arginine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d7fa43de-bc79-5059-b3ca-1a233156f4d1","slug":"slc7a1","display_name":"CAT1 / SLC7A1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full-text methods/results/discussion, Europe PMC PMC9217908.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HEK cells expressing human CAT1, compared with vector controls; radiolabeled substrate uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport competition is distinct from direct NOS inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An endogenous arginine derivative can compete at the entry step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Screening of commonly prescribed drugs for effects on the CAT1-mediated transport of L-arginine and arginine derivatives. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35377022/ · DOI 10.1007/s00726-022-03156-2","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b7cd153b-9e31-525b-9703-679f2bc6aa64","evidence_kind":"source_excerpt","locator":"Lines 30-36","start_line":30,"end_line":36,"excerpt":"## arg-adma-transport\nAn endogenous arginine derivative can compete at the entry step.\nADMA produced a competitive inhibition pattern for CAT1-mediated arginine uptake.\nModel: HEK cells expressing human CAT1, compared with vector controls; radiolabeled substrate uptake.\nLimitations: Transport competition is distinct from direct NOS inhibition.\nEvidence access: Full-text methods/results/discussion, Europe PMC PMC9217908.\nScreening of commonly prescribed drugs for effects on the CAT1-mediated transport of L-arginine and arginine derivatives. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35377022/ · DOI 10.1007/s00726-022-03156-2","model_system":"HEK cells expressing human CAT1, compared with vector controls; radiolabeled substrate uptake.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"ec0e9a3fb72b7485534e4372a074ac7624a399ff962e55690eb462217b5149ec","revision_id":"af027270-5535-51ae-85ff-9c76c518c962","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}