{"id":"f89cdfaf-0578-55ed-baed-a5d7669a65c0","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:plod1-collagen-hydroxylation","predicate":"hydroxylates","statement":"PLOD1 hydroxylates selected collagen-bound lysine residues to hydroxylysine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ce4fdef7-3489-5b8b-abac-5eda798d85d2","mechanism_event_label":"An enzyme adds a hydroxyl group to selected lysines in collagen.","subject":{"id":"95d48931-d1c5-518c-8cbb-4c6ed54b789c","slug":"plod1","display_name":"PLOD1","entity_type_key":"protein"},"object":{"id":"34b151b4-591b-5008-bc84-5f1d610d102b","slug":"collagen-lysine-residues","display_name":"Collagen-bound lysine residues","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"ce4fdef7-3489-5b8b-abac-5eda798d85d2","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:plod1-collagen-hydroxylation-event","event_type":"biochemical_relationship","label":"An enzyme adds a hydroxyl group to selected lysines in collagen.","description":"PLOD1 hydroxylates selected collagen-bound lysine residues to hydroxylysine.","status":"provisional","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"95d48931-d1c5-518c-8cbb-4c6ed54b789c","slug":"plod1","display_name":"PLOD1","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"34b151b4-591b-5008-bc84-5f1d610d102b","slug":"collagen-lysine-residues","display_name":"Collagen-bound lysine residues","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7c465ab2-233e-5401-9706-3492e9ea8f45","slug":"collagen-hydroxylysine-residues","display_name":"Collagen-bound 5-hydroxylysine residues","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human lysyl hydroxylase characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An enzyme adds a hydroxyl group to selected lysines in collagen.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[plod1-1992] Cloning of human lysyl hydroxylase: complete cDNA-derived amino acid sequence and assignment of the gene (PLOD) to chromosome 1p36.3--p36.2 (1992). https://pubmed.ncbi.nlm.nih.gov/1577494/ DOI: 10.1016/0888-7543(92)90202-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"46364448-a08e-570e-acd8-fa1c8a5b5765","evidence_kind":"source_excerpt","locator":"Lines 387-395","start_line":387,"end_line":395,"excerpt":"### plod1-collagen-hydroxylation\nPLOD1 hydroxylates selected collagen-bound lysine residues to hydroxylysine.\nPlain language: An enzyme adds a hydroxyl group to selected lysines in collagen.\nCondition category: normal\norganism: Human\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: Human lysyl hydroxylase characterization.\nlimitations: This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.\n[plod1-1992] Cloning of human lysyl hydroxylase: complete cDNA-derived amino acid sequence and assignment of the gene (PLOD) to chromosome 1p36.3--p36.2 (1992). https://pubmed.ncbi.nlm.nih.gov/1577494/ DOI: 10.1016/0888-7543(92)90202-4","model_system":"Human lysyl hydroxylase characterization.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [plod1-1992] Cloning of human lysyl hydroxylase: complete cDNA-derived amino acid sequence and assignment of the gene (PLOD) to chromosome 1p36.3--p36.2 (1992). https://pubmed.ncbi.nlm.nih.gov/1577494/ DOI: 10.1016/0888-7543(92)90202-4","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}