{"id":"f8645621-0948-5fe8-b08f-d64d0bdaa12f","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:capsaicin-glp1-null","predicate":"no_detected_change_in_recorded_experiment","statement":"Capsaicin did not increase GLP-1 secretion in the tested mouse intestinal and GLUTag cultures.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"6c207adb-942c-5ba7-a105-5853344a61d8","mechanism_event_label":"Capsaicin did not increase GLP-1 secretion in the tested mouse intestinal and GLUTag cultures.","subject":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"object":{"id":"524541e6-4f03-5f43-b02b-fe67675adfe7","slug":"mouse-intestinal-glp1-secretion","display_name":"GLP-1 secretion in mouse intestinal cultures","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6c207adb-942c-5ba7-a105-5853344a61d8","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:capsaicin-glp1-null-event","event_type":"experimental_observation","label":"Capsaicin did not increase GLP-1 secretion in the tested mouse intestinal and GLUTag cultures.","description":"**The GLP-1 experiment did not test allicin.** Allyl isothiocyanate (AITC) and carvacrol increased calcium signaling and GLP-1 secretion in mouse intestinal cultures; antagonism or Trpa1 deletion limited those responses. Glucose-induced responses persisted during TRPA1 blockade. In vivo, oral AITC at 15 mg/kg raised GLP-1 even in Trpa1-null mice; lower doses did not significantly increase it. Capsaicin did not increase secretion in the tested cultures. These findings constrain a proposed allicin–TRPA1–GLP-1 connection, but they do not demonstrate it. AITC is not allicin. [Emery 2015](https://pmc.ncbi.nlm.nih.gov/articles/PMC4375100/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Capsaicin","sequence_order":0,"notes":""},{"entity":{"id":"524541e6-4f03-5f43-b02b-fe67675adfe7","slug":"mouse-intestinal-glp1-secretion","display_name":"GLP-1 secretion in mouse intestinal cultures","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""},{"entity":{"id":"5fb8c3cd-578c-568e-a1f8-bbd7191968c4","slug":"mouse-trpv1","display_name":"Mouse transient receptor potential vanilloid 1 / Trpv1","entity_type_key":"protein"},"role":"candidate receptor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Capsaicin\", \"comparator\": \"Matched basal culture\", \"endpoint\": \"Capsaicin did not increase GLP-1 secretion in the tested mouse intestinal and GLUTag cultures.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Capsaicin 100 nM, two-hour secretion assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No generic TRPV1-to-GLP-1 effect inferred; no allicin exposure in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Capsaicin did not increase GLP-1 secretion in the tested mouse intestinal and GLUTag cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Stimulation of GLP-1 secretion downstream of the ligand-gated ion channel TRPA1. | 2015 | DOI 10.2337/db14-0737 | PMID 25325736 | https://pubmed.ncbi.nlm.nih.gov/25325736/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4375100/ | https://doi.org/10.2337/db14-0737","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 75-75; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3e4e36a9-48a1-5591-9720-d62b88d562c1","evidence_kind":"source_excerpt","locator":"Lines 75-75","start_line":75,"end_line":75,"excerpt":"**The GLP-1 experiment did not test allicin.** Allyl isothiocyanate (AITC) and carvacrol increased calcium signaling and GLP-1 secretion in mouse intestinal cultures; antagonism or Trpa1 deletion limited those responses. Glucose-induced responses persisted during TRPA1 blockade. In vivo, oral AITC at 15 mg/kg raised GLP-1 even in Trpa1-null mice; lower doses did not significantly increase it. Capsaicin did not increase secretion in the tested cultures. These findings constrain a proposed allicin–TRPA1–GLP-1 connection, but they do not demonstrate it. AITC is not allicin. [Emery 2015](https://pmc.ncbi.nlm.nih.gov/articles/PMC4375100/)","model_system":"Capsaicin 100 nM, two-hour secretion assay.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}