{"id":"f85ba1c2-cec4-5056-bbee-d8a050f049dc","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-pendrin-chloride","predicate":"transports","statement":"Expression of human pendrin increased chloride transport in Xenopus oocytes and Sf9 cells, alongside increased iodide transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"92c0cec3-dd5e-555f-885b-c5b893750758","mechanism_event_label":"Pendrin handles chloride as well as iodide.","subject":{"id":"57fef31d-e7e6-5b6f-aee1-77fb94592ce7","slug":"slc26a4","display_name":"Human pendrin / SLC26A4","entity_type_key":"protein"},"object":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"92c0cec3-dd5e-555f-885b-c5b893750758","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-pendrin-chloride-event","event_type":"biochemical_relationship","label":"Pendrin handles chloride as well as iodide.","description":"Expression of human pendrin increased chloride transport in Xenopus oocytes and Sf9 cells, alongside increased iodide transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"transported_anion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"other_transported_anion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"57fef31d-e7e6-5b6f-aee1-77fb94592ce7","slug":"slc26a4","display_name":"Human pendrin / SLC26A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human pendrin expressed in Xenopus oocytes and Sf9 insect cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"PDS cRNA microinjection or recombinant baculovirus expression; concentrations not provided in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This demonstrates shared anion transport capability, not that dietary chloride deficiency blocks iodine repletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein in Xenopus laevis and Spodoptera frugiperda cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Pendrin handles chloride as well as iodide.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-pendrin-halides1999] The Pendred syndrome gene encodes a chloride-iodide transport protein. (1999). https://pubmed.ncbi.nlm.nih.gov/10192399/ DOI: 10.1038/7783","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heterologous cell membranes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"Pendrin is an anion exchanger and the record reports increased transport without naming its direction.","entity":null},{"dimension":"transport_pool","value_text":"the cytosol across the plasma membrane","comparator":null,"unit":null,"notes":"Pendrin is an anion exchanger and the record reports increased transport without naming its direction.","entity":null}],"evidence":[{"id":"b8851b4f-2f0f-5721-a5ae-3672b8925ba0","evidence_kind":"source_excerpt","locator":"Lines 310-321","start_line":310,"end_line":321,"excerpt":"### iodine-trans-pendrin-chloride\nExpression of human pendrin increased chloride transport in Xenopus oocytes and Sf9 cells, alongside increased iodide transport.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Pendrin handles chloride as well as iodide.\norganism: Human protein in Xenopus laevis and Spodoptera frugiperda cells\ntissue_or_cell_type: Heterologous cell membranes\nexperimental_model: Human pendrin expressed in Xenopus oocytes and Sf9 insect cells\nlimitations: This demonstrates shared anion transport capability, not that dietary chloride deficiency blocks iodine repletion.\nexposure: PDS cRNA microinjection or recombinant baculovirus expression; concentrations not provided in abstract.\ncross_nutrient: true\n[iodine-trans-pendrin-halides1999] The Pendred syndrome gene encodes a chloride-iodide transport protein. 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