{"id":"f83eb4f4-96b3-556a-a53c-5f17a7cad4d9","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfite-gpx-time","predicate":"inhibits_after_longer_exposure","statement":"GPx, GST and G6PD activities were unchanged after one hour but inhibited after three hours of sulfite exposure.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"df6d8300-1806-5676-b3e9-cea742dedf7f","mechanism_event_label":"A short experiment and a longer one can show different stages of the same response.","subject":{"id":"914ace7c-9176-53f7-80a9-9ee71d26ea52","slug":"sulfite","display_name":"Sulfite / SO3(2-)","entity_type_key":"ion"},"object":{"id":"424a70bc-a1a7-5e9c-a511-b5ba40fcc793","slug":"rat-gpx-activity","display_name":"Rat cortical glutathione peroxidase enzymes","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"df6d8300-1806-5676-b3e9-cea742dedf7f","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfite-gpx-time-event","event_type":"biochemical_relationship","label":"A short experiment and a longer one can show different stages of the same response.","description":"GPx, GST and G6PD activities were unchanged after one hour but inhibited after three hours of sulfite exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6e1aaed7-9047-533a-9ee4-34126758f7e8","slug":"rat-gst-activity","display_name":"Rat cortical glutathione S-transferase enzymes","entity_type_key":"protein_family"},"role":"affected activity","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f60a56fa-95b3-570d-b219-3f690b62dd05","slug":"rat-cortical-g6pd-activity","display_name":"Rat cortical glucose-6-phosphate dehydrogenase activity","entity_type_key":"cellular_process"},"role":"affected activity","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"914ace7c-9176-53f7-80a9-9ee71d26ea52","slug":"sulfite","display_name":"Sulfite / SO3(2-)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"424a70bc-a1a7-5e9c-a511-b5ba40fcc793","slug":"rat-gpx-activity","display_name":"Rat cortical glutathione peroxidase enzymes","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/25777939.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1768480178724867d4c7a98f723cfd1a0010f7973eae4a4baea3d1261a06fdd2\", \"start_char\": 0, \"end_char\": 2017, \"text_sha256\": \"1768480178724867d4c7a98f723cfd1a0010f7973eae4a4baea3d1261a06fdd2\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Sulfite/thiosulfate incubation of rat cortical slices and cell-free GSSG","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"One- versus three-hour incubations; 10 micromolar sulfite redox observations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Concentration, duration and preparation matter; GPx activity is not evidence of selenium depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus; cell-free comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A short experiment and a longer one can show different stages of the same response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p25777939] In vitro evidence that sulfite impairs glutamatergic neurotransmission and inhibits glutathione metabolism-related enzymes in rat cerebral cortex. (2015). https://pubmed.ncbi.nlm.nih.gov/25777939/ DOI: 10.1016/j.ijdevneu.2015.03.005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cerebral cortex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"7a9d57c2-6b8a-53a9-9f9e-6ec61107c079","evidence_kind":"source_excerpt","locator":"Lines 1418-1429","start_line":1418,"end_line":1429,"excerpt":"### mo-sulfite-gpx-time\nGPx, GST and G6PD activities were unchanged after one hour but inhibited after three hours of sulfite exposure.\nCondition category: machinery_impairment\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A short experiment and a longer one can show different stages of the same response.\norganism: Rattus norvegicus; cell-free comparison\ntissue_or_cell_type: Cerebral cortex\nexperimental_model: Sulfite/thiosulfate incubation of rat cortical slices and cell-free GSSG\nlimitations: Concentration, duration and preparation matter; GPx activity is not evidence of selenium depletion.\nexposure: One- versus three-hour incubations; 10 micromolar sulfite redox observations\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/25777939.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1768480178724867d4c7a98f723cfd1a0010f7973eae4a4baea3d1261a06fdd2\", \"start_char\": 0, \"end_char\": 2017, \"text_sha256\": \"1768480178724867d4c7a98f723cfd1a0010f7973eae4a4baea3d1261a06fdd2\"}\n[mo-p25777939] In vitro evidence that sulfite impairs glutamatergic neurotransmission and inhibits glutathione metabolism-related enzymes in rat cerebral cortex. (2015). https://pubmed.ncbi.nlm.nih.gov/25777939/ DOI: 10.1016/j.ijdevneu.2015.03.005","model_system":"Sulfite/thiosulfate incubation of rat cortical slices and cell-free GSSG","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p25777939] In vitro evidence that sulfite impairs glutamatergic neurotransmission and inhibits glutathione metabolism-related enzymes in rat cerebral cortex. 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