{"id":"f811d5d8-08f3-5101-9e6d-cf7c54c6c112","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-mitochondrial-state","predicate":"has_concentration_dependent_effect_on","statement":"Agmatine induced permeability transition at lower tested concentrations but inhibited calcium/phosphate-associated swelling at higher concentrations in isolated rat liver mitochondria.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a2b8a348-e52a-55fc-80b7-53673199408a","mechanism_event_label":"A compound can change direction across concentrations and assay endpoints.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"f23ffc97-fbd0-50a7-9570-40a65612a1a4","slug":"rat-liver-agmatine-mitochondrial-permeability","display_name":"Rat liver mitochondrial permeability during agmatine exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a2b8a348-e52a-55fc-80b7-53673199408a","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-mitochondrial-state-event","event_type":"observed_relationship","label":"A compound can change direction across concentrations and assay endpoints.","description":"Agmatine induced permeability transition at lower tested concentrations but inhibited calcium/phosphate-associated swelling at higher concentrations in isolated rat liver mitochondria.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f23ffc97-fbd0-50a7-9570-40a65612a1a4","slug":"rat-liver-agmatine-mitochondrial-permeability","display_name":"Rat liver mitochondrial permeability during agmatine exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b28dfc54-a5ef-5f9e-ac1f-890b68e58dc3","slug":"inorganic-phosphate","display_name":"Inorganic phosphate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat liver mitochondria; swelling and protein-release assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Numerical concentration ranges are not provided in the accessed abstract; no human protective threshold is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A compound can change direction across concentrations and assay endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Involvement of the biogenic active amine agmatine in mitochondrial membrane permeabilization and release of pro-apoptotic factors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/31654209/ · DOI 10.1007/s00726-019-02791-6","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9e4868d5-6428-51cc-a2d9-dfea32dad679","evidence_kind":"source_excerpt","locator":"Lines 388-394","start_line":388,"end_line":394,"excerpt":"## agmatine-sulfate-mitochondrial-state\nA compound can change direction across concentrations and assay endpoints.\nAgmatine induced permeability transition at lower tested concentrations but inhibited calcium/phosphate-associated swelling at higher concentrations in isolated rat liver mitochondria.\nModel: Isolated rat liver mitochondria; swelling and protein-release assays.\nLimitations: Numerical concentration ranges are not provided in the accessed abstract; no human protective threshold is inferred.\nEvidence access: Primary abstract\nInvolvement of the biogenic active amine agmatine in mitochondrial membrane permeabilization and release of pro-apoptotic factors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/31654209/ · DOI 10.1007/s00726-019-02791-6","model_system":"Isolated rat liver mitochondria; swelling and protein-release assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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