{"id":"f7fd9be8-83d3-53bf-bc6a-80063aec4971","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-oxidant-coalation","predicate":"induces","statement":"Hydrogen peroxide exposure induced DTT-sensitive protein CoAlation in isolated rat cardiomyocytes and perfused rat hearts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a2a3bba1-af71-5129-abc3-e392e84d3663","mechanism_event_label":"Oxidative stress caused CoA to form reversible bonds with protein cysteines.","subject":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"object":{"id":"9cb713ef-3378-5882-934c-bd037e5edadc","slug":"protein-coalation","display_name":"Protein cysteine CoAlation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a2a3bba1-af71-5129-abc3-e392e84d3663","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-oxidant-coalation-event","event_type":"biochemical_relationship","label":"Oxidative stress caused CoA to form reversible bonds with protein cysteines.","description":"Hydrogen peroxide exposure induced DTT-sensitive protein CoAlation in isolated rat cardiomyocytes and perfused rat hearts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"protein_modifying_thiol","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1f79fec8-f7a0-5537-a03a-e74fb90b6c0b","slug":"protein-cysteine-coa-disulfide","display_name":"Protein cysteine–CoA mixed disulfides","entity_type_key":"protein_state"},"role":"modified_product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9cb713ef-3378-5882-934c-bd037e5edadc","slug":"protein-coalation","display_name":"Protein cysteine CoAlation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text lines 105–107; Fig. 1B–D","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary cardiomyocyte exposure and Langendorff-perfused rat hearts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cardiomyocytes: 1–100 micromolar H2O2, 30 minutes; perfused hearts: 100 micromolar H2O2, 20 minutes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"DTT sensitivity supports mixed disulfides. Antioxidant protection or improved organ function was not established by this endpoint. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Oxidative stress caused CoA to form reversible bonds with protein cysteines.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. (2017). https://pubmed.ncbi.nlm.nih.gov/28341808/ DOI: 10.1042/bcj20170129","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adult cardiomyocytes and isolated heart","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2f493da3-0853-5154-8a8e-c15e21ce06d3","evidence_kind":"source_excerpt","locator":"Lines 1047-1059","start_line":1047,"end_line":1059,"excerpt":"### b5-met-oxidant-coalation\nHydrogen peroxide exposure induced DTT-sensitive protein CoAlation in isolated rat cardiomyocytes and perfused rat hearts.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Oxidative stress caused CoA to form reversible bonds with protein cysteines.\norganism: Rattus norvegicus\ntissue_or_cell_type: Adult cardiomyocytes and isolated heart\nexperimental_model: Primary cardiomyocyte exposure and Langendorff-perfused rat hearts\nlimitations: DTT sensitivity supports mixed disulfides. Antioxidant protection or improved organ function was not established by this endpoint. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Cardiomyocytes: 1–100 micromolar H2O2, 30 minutes; perfused hearts: 100 micromolar H2O2, 20 minutes.\ncross_nutrient: false\nevidence_location: Full text lines 105–107; Fig. 1B–D\n[b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. (2017). https://pubmed.ncbi.nlm.nih.gov/28341808/ DOI: 10.1042/bcj20170129","model_system":"Primary cardiomyocyte exposure and Langendorff-perfused rat hearts","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. (2017). https://pubmed.ncbi.nlm.nih.gov/28341808/ DOI: 10.1042/bcj20170129","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}