{"id":"f7e5c355-b676-5cf0-8aab-04ca42ef301a","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-stat3-engagement","predicate":"changes_thermal_stability_of","statement":"Docking and cellular thermal-shift experiments supported STAT3 target engagement by cucurbitacin B, alongside reduced STAT3 phosphorylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"445d2b70-acbb-512f-81d8-44cfceb7513a","mechanism_event_label":"The paper tested target engagement as well as signaling.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"445d2b70-acbb-512f-81d8-44cfceb7513a","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-stat3-engagement-event","event_type":"observed_relationship","label":"The paper tested target engagement as well as signaling.","description":"Docking and cellular thermal-shift experiments supported STAT3 target engagement by cucurbitacin B, alongside reduced STAT3 phosphorylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human NSCLC study with docking and CETSA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"CETSA is not purified binding kinetics or proof that STAT3 is the sole or selective target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The paper tested target engagement as well as signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B targets STAT3 to induce ferroptosis in non-small cell lung cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38950838/ · DOI 10.1016/j.ejphar.2024.176805","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"889e960a-6f6f-54b6-b73a-cd8e2333ad37","evidence_kind":"source_excerpt","locator":"Lines 268-274","start_line":268,"end_line":274,"excerpt":"## cucurbitacin-b-stat3-engagement\nThe paper tested target engagement as well as signaling.\nDocking and cellular thermal-shift experiments supported STAT3 target engagement by cucurbitacin B, alongside reduced STAT3 phosphorylation.\nModel: Human NSCLC study with docking and CETSA.\nLimitations: CETSA is not purified binding kinetics or proof that STAT3 is the sole or selective target.\nEvidence access: Primary abstract\nCucurbitacin B targets STAT3 to induce ferroptosis in non-small cell lung cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38950838/ · DOI 10.1016/j.ejphar.2024.176805","model_system":"Human NSCLC study with docking and CETSA.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}