{"id":"f7d66243-9587-5e5c-b376-27f783f0dc8b","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-rgs-inhibition","predicate":"reduces_tested","statement":"Glycine slowed G-alpha-o deactivation only when GPR158 was coexpressed with RGS7/G-beta5, supporting inhibition of the complex's GTPase-accelerating activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1780be02-8c14-589b-b47c-cb1f19e57dd2","mechanism_event_label":"Receptor binding changes how quickly another signaling switch is turned off.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"ad57ab75-a572-5b0f-b6a9-0374f57d4a9d","slug":"gpr158-rgs7-gnb5-assay","display_name":"GPR158-associated RGS7/G-beta5 assay complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"1780be02-8c14-589b-b47c-cb1f19e57dd2","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-rgs-inhibition-event","event_type":"observed_relationship","label":"Receptor binding changes how quickly another signaling switch is turned off.","description":"Glycine slowed G-alpha-o deactivation only when GPR158 was coexpressed with RGS7/G-beta5, supporting inhibition of the complex's GTPase-accelerating activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ad57ab75-a572-5b0f-b6a9-0374f57d4a9d","slug":"gpr158-rgs7-gnb5-assay","display_name":"GPR158-associated RGS7/G-beta5 assay complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c1793375-e328-5a81-940b-046c14ad45fe","slug":"gpr158-recombinant-assay","display_name":"Recombinant GPR158 in the 2023 binding/signaling assays","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"18e33eb9-a330-5b78-9cc5-f57dcbc6571c","slug":"rgs7-recombinant-assay","display_name":"Recombinant RGS7 in the 2023 GPR158 assays","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"73e7d4e6-1c48-5d58-9bcd-f975e12c4edf","slug":"gnb5-recombinant-assay","display_name":"Recombinant G-beta5 in the 2023 GPR158 assays","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant cellular BRET assays, including 100 micromolar glycine comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Functional coupling does not prove every intervening conformational step or a clinical mood effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Receptor binding changes how quickly another signaling switch is turned off.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f12c13a7-6da1-5e46-ae7c-7fead58f8739","evidence_kind":"source_excerpt","locator":"Lines 226-232","start_line":226,"end_line":232,"excerpt":"## glycine-gpr158-rgs-inhibition\nReceptor binding changes how quickly another signaling switch is turned off.\nGlycine slowed G-alpha-o deactivation only when GPR158 was coexpressed with RGS7/G-beta5, supporting inhibition of the complex's GTPase-accelerating activity.\nModel: Recombinant cellular BRET assays, including 100 micromolar glycine comparisons.\nLimitations: Functional coupling does not prove every intervening conformational step or a clinical mood effect.\nEvidence access: Primary full text\nOrphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","model_system":"Recombinant cellular BRET assays, including 100 micromolar glycine comparisons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}