{"id":"f7cece87-42a6-5140-bb07-6bf35ed7ee5c","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:cldn16-rnai-magnesium-loss","predicate":"increases","statement":"Claudin-16 RNAi mice developed renal magnesium wasting.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"cade0669-1ed8-5f6d-88fd-c9dcd6ceb41f","mechanism_event_label":"Reducing this tight-junction component made the kidneys lose magnesium.","subject":{"id":"f7b8e03e-63ac-5f82-ad8d-e811391a8e29","slug":"mouse-cldn16-rnai-genotype","display_name":"Claudin-16 RNAi mouse genotype","entity_type_key":"gene"},"object":{"id":"a298b414-0e07-568d-a469-d8aa3e8b8c14","slug":"urinary-magnesium-excretion","display_name":"Urinary magnesium excretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cade0669-1ed8-5f6d-88fd-c9dcd6ceb41f","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:cldn16-rnai-magnesium-loss-event","event_type":"biochemical_relationship","label":"Reducing this tight-junction component made the kidneys lose magnesium.","description":"Claudin-16 RNAi mice developed renal magnesium wasting.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b9e564ce-7834-5815-a22b-0aa555a7bfe4","slug":"cldn16","display_name":"Claudin-16","entity_type_key":"protein"},"role":"depleted-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"lost-ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"co-lost-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f7b8e03e-63ac-5f82-ad8d-e811391a8e29","slug":"mouse-cldn16-rnai-genotype","display_name":"Claudin-16 RNAi mouse genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a298b414-0e07-568d-a469-d8aa3e8b8c14","slug":"urinary-magnesium-excretion","display_name":"Urinary magnesium excretion","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Calcium wasting accompanied magnesium loss after shared tight-junction impairment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence-system","value_text":"Transgenic Cldn16 RNAi with renal mineral phenotyping","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transgenic Cldn16 RNAi with renal mineral phenotyping","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This organismal endpoint accompanies selectivity changes; the experiment does not isolate every intervening transport step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Reducing this tight-junction component made the kidneys lose magnesium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hou-2007-cldn16] Transgenic RNAi depletion of claudin-16 and the renal handling of magnesium. (2007). https://pubmed.ncbi.nlm.nih.gov/17442678/ DOI: 10.1074/jbc.M700632200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Kidney; urine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney; urine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"38d686f3-6000-56c3-90ae-a27c13229e07","evidence_kind":"source_excerpt","locator":"Lines 1111-1123","start_line":1111,"end_line":1123,"excerpt":"### cldn16-rnai-magnesium-loss\nClaudin-16 RNAi mice developed renal magnesium wasting.\nCondition category: machinery_impairment\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Reducing this tight-junction component made the kidneys lose magnesium.\norganism: Mouse\ntissue_or_cell_type: Kidney; urine\nexperimental_model: Transgenic Cldn16 RNAi with renal mineral phenotyping\nlimitations: This organismal endpoint accompanies selectivity changes; the experiment does not isolate every intervening transport step.\ncross_nutrient: Calcium wasting accompanied magnesium loss after shared tight-junction impairment.\nevidence-system: Transgenic Cldn16 RNAi with renal mineral phenotyping\ntissue: Kidney; urine\n[hou-2007-cldn16] Transgenic RNAi depletion of claudin-16 and the renal handling of magnesium. 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