{"id":"f78559da-fa25-5a06-ae82-cd36dcb0a1bc","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-nox2-null","predicate":"not_required_for","statement":"Cybb/gp91phox-deficient macrophages retained silica-triggered inflammasome responses.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"8bf7b4a9-5b46-5199-9129-f25c0e652282","mechanism_event_label":"The phagocyte NADPH oxidase burst was not obligatory in this model.","subject":{"id":"a059a624-d2fd-5592-b41c-370798c41179","slug":"mouse-cybb","display_name":"Mouse CYBB / gp91phox","entity_type_key":"protein"},"object":{"id":"14c5e985-d063-5a24-bdab-1883f3fa449c","slug":"mouse-silica-inflammasome-response","display_name":"Mouse silica inflammasome response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8bf7b4a9-5b46-5199-9129-f25c0e652282","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-nox2-null-event","event_type":"observed_relationship","label":"The phagocyte NADPH oxidase burst was not obligatory in this model.","description":"Cybb/gp91phox-deficient macrophages retained silica-triggered inflammasome responses.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a059a624-d2fd-5592-b41c-370798c41179","slug":"mouse-cybb","display_name":"Mouse CYBB / gp91phox","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"14c5e985-d063-5a24-bdab-1883f3fa449c","slug":"mouse-silica-inflammasome-response","display_name":"Mouse silica inflammasome response","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b2d4231b-3893-5d4e-8b52-d0effdaa26c9","slug":"crystalline-silica","display_name":"Crystalline silica / quartz particles","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ae25f14e-2a54-5094-9952-a97b718f94a3","slug":"mouse-nlrp3","display_name":"Mouse NLRP3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse macrophage genetic comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not exclude every ROS source or establish NADPH depletion. Correction record: A 2026 author correction replaces an erroneously duplicated lower-left Fig. 8c micrograph with the retrieved original. This is a figure correction, not a scientific contradiction or retraction. https://www.nature.com/articles/s41590-026-02468-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The phagocyte NADPH oxidase burst was not obligatory in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18604214/ · DOI 10.1038/ni.1631","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"001da2f8-ddfe-5613-b7ac-2937ac33d805","evidence_kind":"source_excerpt","locator":"Lines 456-462","start_line":456,"end_line":462,"excerpt":"## silica-nox2-null\nThe phagocyte NADPH oxidase burst was not obligatory in this model.\nCybb/gp91phox-deficient macrophages retained silica-triggered inflammasome responses.\nModel: Mouse macrophage genetic comparison.\nLimitations: Does not exclude every ROS source or establish NADPH depletion. Correction record: A 2026 author correction replaces an erroneously duplicated lower-left Fig. 8c micrograph with the retrieved original. This is a figure correction, not a scientific contradiction or retraction. https://www.nature.com/articles/s41590-026-02468-9\nEvidence access: Primary full text\nSilica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18604214/ · DOI 10.1038/ni.1631","model_system":"Mouse macrophage genetic comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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