{"id":"f7693bb8-3211-52f0-8f7b-bb6addf1ae74","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-ck2-crystal","predicate":"reported_relationship","statement":"Crystallography placed apigenin in the catalytic pocket of Zea mays CK2, with ATP-competitive inhibition reported in the study.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"9d7fc6e7-ab1f-5013-9125-acc6fe0c94c9","mechanism_event_label":"Crystallography placed apigenin in the catalytic pocket of Zea mays CK2, with ATP-competitive inhibition reported in the study.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"90862c8e-edce-5338-88da-ed73d1899a62","slug":"zea-mays-ck2-catalytic","display_name":"Zea mays CK2 catalytic subunit","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"9d7fc6e7-ab1f-5013-9125-acc6fe0c94c9","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-ck2-crystal-event","event_type":"biochemical_relationship","label":"Crystallography placed apigenin in the catalytic pocket of Zea mays CK2, with ATP-competitive inhibition reported in the study.","description":"### api-ck2-crystal\n\nCrystallography placed apigenin in the catalytic pocket of Zea mays CK2, with ATP-competitive inhibition reported in the study.\nThe structure is not human CK2. The low-micromolar/submicromolar kinase results are not a measured oral tissue exposure.\nModel and exposure: Primary abstract plus PDB 4DGM, 1.65 Å; maize catalytic subunit.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibition of protein kinase CK2 by flavonoids and tyrphostins. A structural insight. | 2012 | https://pubmed.ncbi.nlm.nih.gov/22794353/ | https://doi.org/10.1021/bi300531c | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"90862c8e-edce-5338-88da-ed73d1899a62","slug":"zea-mays-ck2-catalytic","display_name":"Zea mays CK2 catalytic subunit","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary abstract plus PDB 4DGM, 1.65 Å; maize catalytic subunit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Crystallography placed apigenin in the catalytic pocket of Zea mays CK2, with ATP-competitive inhibition reported in the study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of protein kinase CK2 by flavonoids and tyrphostins. A structural insight. | https://pubmed.ncbi.nlm.nih.gov/22794353/ | https://doi.org/10.1021/bi300531c","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-ck2-crystal; The structure is not human CK2. The low-micromolar/submicromolar kinase results are not a measured oral tissue exposure.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dcd1b745-dc75-57ed-b28d-e6ee2fc3cb1d","evidence_kind":"source_excerpt","locator":"Lines 584-590","start_line":584,"end_line":590,"excerpt":"### api-ck2-crystal\n\nCrystallography placed apigenin in the catalytic pocket of Zea mays CK2, with ATP-competitive inhibition reported in the study.\nThe structure is not human CK2. The low-micromolar/submicromolar kinase results are not a measured oral tissue exposure.\nModel and exposure: Primary abstract plus PDB 4DGM, 1.65 Å; maize catalytic subunit.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibition of protein kinase CK2 by flavonoids and tyrphostins. A structural insight. | 2012 | https://pubmed.ncbi.nlm.nih.gov/22794353/ | https://doi.org/10.1021/bi300531c | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Primary abstract plus PDB 4DGM, 1.65 Å; maize catalytic subunit.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}