{"id":"f7610f5d-358d-578d-aa6a-045769beb9ce","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-salmonella-outcome-null","predicate":"does_not_prevent","statement":"Despite blocking ERK/JNK activation, bromelain did not prevent Salmonella invasion or the fall in Caco-2 monolayer resistance.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"5ce741ca-824d-58ac-b326-3fb5d8de53d1","mechanism_event_label":"Despite blocking ERK/JNK activation, bromelain did not prevent Salmonella invasion or the fall in Caco-2 monolayer resistance.","subject":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"object":{"id":"238501bd-7931-5a18-8ae2-f89c48c2522b","slug":"salmonella-caco2-invasion-and-barrier-loss","display_name":"Salmonella invasion and barrier-resistance loss in Caco-2 monolayers","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5ce741ca-824d-58ac-b326-3fb5d8de53d1","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-salmonella-outcome-null-event","event_type":"observed_relationship","label":"Despite blocking ERK/JNK activation, bromelain did not prevent Salmonella invasion or the fall in Caco-2 monolayer resistance.","description":"Despite blocking ERK/JNK activation, bromelain did not prevent Salmonella invasion or the fall in Caco-2 monolayer resistance.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"238501bd-7931-5a18-8ae2-f89c48c2522b","slug":"salmonella-caco2-invasion-and-barrier-loss","display_name":"Salmonella invasion and barrier-resistance loss in Caco-2 monolayers","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8708f3a5-4319-552b-9a35-0c8563e4331d","slug":"salmonella-typhimurium","display_name":"Salmonella enterica serovar Typhimurium","entity_type_key":"organism"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Bromelain with Salmonella Typhimurium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute infection assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 intestinal epithelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This null finding prevents kinase inhibition from being presented as protection against infection or barrier injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Bromelain chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"}},{"dimension":"organism","value_text":"Human Caco-2 intestinal epithelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Despite blocking ERK/JNK activation, bromelain did not prevent Salmonella invasion or the fall in Caco-2 monolayer resistance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Proteolytic inhibition of Salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases ERK and JNK in cultured human intestinal cells. (2002). https://pubmed.ncbi.nlm.nih.gov/11748167/ DOI: 10.1128/IAI.70.1.86-95.2002","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Bacterial invasion and transepithelial resistance","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4047de72-3909-5826-8d7f-e022f2c2eb8b","evidence_kind":"source_excerpt","locator":"Lines 88-97","start_line":88,"end_line":97,"excerpt":"## bromelain-salmonella-outcome-null\nDespite blocking ERK/JNK activation, bromelain did not prevent Salmonella invasion or the fall in Caco-2 monolayer resistance.\nModel/species: Human Caco-2 intestinal epithelial cells\nTissue/system: Bacterial invasion and transepithelial resistance\nExposure: Bromelain with Salmonella Typhimurium\nRoute: In vitro\nDuration: Acute infection assay\nLimits: This null finding prevents kinase inhibition from being presented as protection against infection or barrier injury.\nPrimary reference: Proteolytic inhibition of Salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases ERK and JNK in cultured human intestinal cells. (2002). https://pubmed.ncbi.nlm.nih.gov/11748167/ DOI: 10.1128/IAI.70.1.86-95.2002\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Human Caco-2 intestinal epithelial cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9d363b4e-f438-5664-9d74-99c2e359391d","stable_key":"import-773f772e-bf8e-5e52-a2a2-6dd694d67f5d","title":"Bromelain: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"b328dbb8082bea612bb2c40d6ff41fa670f6e62f714bd014c90627fcab86e95b","revision_id":"1c10d22e-ecdf-56ef-b820-bb21182a9577","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}