{"id":"f7507f1f-deca-52bb-b9af-4228725f5e84","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-ddo-peroxisome","predicate":"localizes_to","statement":"Subcellular fractionation localized D-aspartate oxidase activity to peroxisomes in human liver.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2842f8a6-1318-532b-bd44-fa2df2bb45fb","mechanism_event_label":"Clearance chemistry is organized within a cellular compartment.","subject":{"id":"17871951-099f-5a48-832e-ff9cb1c65755","slug":"ddo","display_name":"Human D-aspartate oxidase / DDO","entity_type_key":"protein"},"object":{"id":"4f5ca28f-4cdb-52b7-b9a7-5a13cdf74bbc","slug":"human-liver-peroxisomes","display_name":"Human liver peroxisomes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2842f8a6-1318-532b-bd44-fa2df2bb45fb","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-ddo-peroxisome-event","event_type":"observed_relationship","label":"Clearance chemistry is organized within a cellular compartment.","description":"Subcellular fractionation localized D-aspartate oxidase activity to peroxisomes in human liver.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"17871951-099f-5a48-832e-ff9cb1c65755","slug":"ddo","display_name":"Human D-aspartate oxidase / DDO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4f5ca28f-4cdb-52b7-b9a7-5a13cdf74bbc","slug":"human-liver-peroxisomes","display_name":"Human liver peroxisomes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human liver fractionation; rat liver also studied.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The study did not find a significant oxidase deficiency in its Zellweger liver samples; peroxisomal disease is not automatically DDO deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Clearance chemistry is organized within a cellular compartment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"D-aspartate oxidase, a peroxisomal enzyme in liver of rat and man. · 1991 · https://pubmed.ncbi.nlm.nih.gov/1991137/ · DOI 10.1016/0304-4165(91)90203-s","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"47a3deef-0342-587b-8edf-dfdf34150b08","evidence_kind":"source_excerpt","locator":"Lines 88-94","start_line":88,"end_line":94,"excerpt":"## d-aspartate-ddo-peroxisome\nClearance chemistry is organized within a cellular compartment.\nSubcellular fractionation localized D-aspartate oxidase activity to peroxisomes in human liver.\nModel: Human liver fractionation; rat liver also studied.\nLimitations: The study did not find a significant oxidase deficiency in its Zellweger liver samples; peroxisomal disease is not automatically DDO deficiency.\nEvidence access: Primary abstract\nD-aspartate oxidase, a peroxisomal enzyme in liver of rat and man. · 1991 · https://pubmed.ncbi.nlm.nih.gov/1991137/ · DOI 10.1016/0304-4165(91)90203-s","model_system":"Human liver fractionation; rat liver also studied.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0a86a1008edb8a69dcc79409e76e3fd5ce59b0747be8cae8f21e43f871e76248","revision_id":"a1de4b66-263d-5452-8ed8-df853afba030","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}