{"id":"f7446ac0-5f5e-5f5e-b8f7-a2a2db35a7fc","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-parent-pk-null","predicate":"no_detected_change_in_recorded_comparison","statement":"After ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"1bd3ed97-2cc6-53b8-8972-634d9bdaa640","mechanism_event_label":"After ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"a2d39504-5f45-5acc-830d-89c73892b358","slug":"apigenin-human-plasma-parent","display_name":"Unconjugated apigenin measured in human plasma","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1bd3ed97-2cc6-53b8-8972-634d9bdaa640","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-parent-pk-null-event","event_type":"experimental_observation","label":"After ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.","description":"### api-parent-pk-null\n\nAfter ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.\nTable 3 and section 3.3.1. Detection was sampling- and assay-dependent, not proof of zero absorption.\nModel and exposure: Three healthy men; single aglycone capsule contents in hot water; about 51.6 mg calculated from molecular mass.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Absorption, distribution, metabolism and excretion of apigenin and its glycosides in healthy male adults. | 2022 | https://pubmed.ncbi.nlm.nih.gov/35452808/ | https://doi.org/10.1016/j.freeradbiomed.2022.04.007 | Access: Full original primary-paper PDF, Methods, Results and Tables 1–7 reviewed; authentic-standard HPLC-MS analysis. PDF obtained from a public mirror; DOI and article identity verified.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"191 micromoles oral apigenin","sequence_order":0,"notes":""},{"entity":{"id":"a2d39504-5f45-5acc-830d-89c73892b358","slug":"apigenin-human-plasma-parent","display_name":"Unconjugated apigenin measured in human plasma","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full original primary-paper PDF, Methods, Results and Tables 1–7 reviewed; authentic-standard HPLC-MS analysis. PDF obtained from a public mirror; DOI and article identity verified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"191 micromoles oral apigenin\", \"comparator\": \"Predose plasma\", \"endpoint\": \"After ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Three healthy men; single aglycone capsule contents in hot water; about 51.6 mg calculated from molecular mass.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"After ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Absorption, distribution, metabolism and excretion of apigenin and its glycosides in healthy male adults. | https://pubmed.ncbi.nlm.nih.gov/35452808/ | https://doi.org/10.1016/j.freeradbiomed.2022.04.007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-parent-pk-null; Table 3 and section 3.3.1. Detection was sampling- and assay-dependent, not proof of zero absorption.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b40d02ff-2f63-5db5-859a-546c831faf1a","evidence_kind":"source_excerpt","locator":"Lines 29-35","start_line":29,"end_line":35,"excerpt":"### api-parent-pk-null\n\nAfter ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.\nTable 3 and section 3.3.1. Detection was sampling- and assay-dependent, not proof of zero absorption.\nModel and exposure: Three healthy men; single aglycone capsule contents in hot water; about 51.6 mg calculated from molecular mass.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Absorption, distribution, metabolism and excretion of apigenin and its glycosides in healthy male adults. | 2022 | https://pubmed.ncbi.nlm.nih.gov/35452808/ | https://doi.org/10.1016/j.freeradbiomed.2022.04.007 | Access: Full original primary-paper PDF, Methods, Results and Tables 1–7 reviewed; authentic-standard HPLC-MS analysis. PDF obtained from a public mirror; DOI and article identity verified.","model_system":"Three healthy men; single aglycone capsule contents in hot water; about 51.6 mg calculated from molecular mass.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[{"id":"fc75d6fc-2618-5460-9400-ef895db2036f","relation_type":"limits_scope_of","notes":"A cell-free micromolar IC50 does not establish exposure at a human tissue target after this oral preparation.","source_claim_id":"f7446ac0-5f5e-5f5e-b8f7-a2a2db35a7fc","source_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-parent-pk-null","source_statement":"After ingestion of 191 micromoles of apigenin, neither parent nor the targeted conjugates were detected in plasma at 0, 2, 4 or 6 hours.","target_claim_id":"9cbf5da4-e7eb-5646-98f0-4ab7135d5e97","target_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-human-cd38-nadase","target_statement":"Apigenin inhibited soluble human CD38 NADase activity with IC50 10.3 ± 2.4 micromolar."}],"conflicts":[],"corrections":[],"research":null}