{"id":"f73ae6d2-57b4-57fc-800e-e8eead6ab087","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-clostridial-tryptamine","predicate":"decarboxylates_to","statement":"Purified C. sporogenes CLOSPO_02083 catalyzed tryptophan decarboxylation to tryptamine as a PLP-dependent enzyme.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b6d72512-e0ef-5fe2-b408-73a68a420a68","mechanism_event_label":"A bacterial B6-dependent enzyme sends tryptophan down another branch.","subject":{"id":"66f33ae7-add9-5f93-ba73-55efdd6a9bd5","slug":"c-sporogenes-clospo-02083","display_name":"Clostridium sporogenes CLOSPO_02083 tryptophan decarboxylase","entity_type_key":"protein"},"object":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b6d72512-e0ef-5fe2-b408-73a68a420a68","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-clostridial-tryptamine-event","event_type":"observed_relationship","label":"A bacterial B6-dependent enzyme sends tryptophan down another branch.","description":"Purified C. sporogenes CLOSPO_02083 catalyzed tryptophan decarboxylation to tryptamine as a PLP-dependent enzyme.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"66f33ae7-add9-5f93-ba73-55efdd6a9bd5","slug":"c-sporogenes-clospo-02083","display_name":"Clostridium sporogenes CLOSPO_02083 tryptophan decarboxylase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, enzyme identification and biochemical assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant bacterial enzyme biochemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish that B6 supplementation increases this product in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A bacterial B6-dependent enzyme sends tryptophan down another branch.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Discovery and characterization of gut microbiota decarboxylases that can produce the neurotransmitter tryptamine. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25263219/ · DOI 10.1016/j.chom.2014.09.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4087f250-0774-5d62-aeaf-5b1ca8e97f38","evidence_kind":"source_excerpt","locator":"Lines 546-552","start_line":546,"end_line":552,"excerpt":"## tryptophan-clostridial-tryptamine\nA bacterial B6-dependent enzyme sends tryptophan down another branch.\nPurified C. sporogenes CLOSPO_02083 catalyzed tryptophan decarboxylation to tryptamine as a PLP-dependent enzyme.\nModel: Recombinant bacterial enzyme biochemistry.\nLimitations: Does not establish that B6 supplementation increases this product in humans.\nEvidence access: Primary full text, enzyme identification and biochemical assays\nDiscovery and characterization of gut microbiota decarboxylases that can produce the neurotransmitter tryptamine. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25263219/ · DOI 10.1016/j.chom.2014.09.001","model_system":"Recombinant bacterial enzyme biochemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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