{"id":"f7015e24-5726-5efd-a9e5-a3d82534bae3","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rpe-catabolism-block","predicate":"supports","statement":"Blocking proline catabolism in human RPE impaired glucose metabolism and glutathione production.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0b6c7c8a-1308-5571-ba2f-2a9e37c86516","mechanism_event_label":"A block in proline use can affect antioxidant production indirectly.","subject":{"id":"5e13a254-f92e-5317-abb5-2c683b178d39","slug":"prodh","display_name":"Human proline dehydrogenase / PRODH","entity_type_key":"protein"},"object":{"id":"9291602a-797b-5610-81e1-fdd66dd72932","slug":"human-rpe-glucose-gsh-metabolism","display_name":"Glucose metabolism and glutathione production in human RPE","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0b6c7c8a-1308-5571-ba2f-2a9e37c86516","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rpe-catabolism-block-event","event_type":"observed_relationship","label":"A block in proline use can affect antioxidant production indirectly.","description":"Blocking proline catabolism in human RPE impaired glucose metabolism and glutathione production.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5e13a254-f92e-5317-abb5-2c683b178d39","slug":"prodh","display_name":"Human proline dehydrogenase / PRODH","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9291602a-797b-5610-81e1-fdd66dd72932","slug":"human-rpe-glucose-gsh-metabolism","display_name":"Glucose metabolism and glutathione production in human RPE","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human RPE culture with pharmacological catabolism inhibition and metabolite measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological specificity and culture conditions limit inference; no human blood-proline threshold is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A block in proline use can affect antioxidant production indirectly.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Proline mediates metabolic communication between retinal pigment epithelial cells and the retina. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31110046/ · DOI 10.1074/jbc.RA119.007983","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"03f34520-00f9-5091-bac7-33c9794f6628","evidence_kind":"source_excerpt","locator":"Lines 318-324","start_line":318,"end_line":324,"excerpt":"## l-proline-rpe-catabolism-block\nA block in proline use can affect antioxidant production indirectly.\nBlocking proline catabolism in human RPE impaired glucose metabolism and glutathione production.\nModel: Human RPE culture with pharmacological catabolism inhibition and metabolite measurements.\nLimitations: Pharmacological specificity and culture conditions limit inference; no human blood-proline threshold is established.\nEvidence access: Primary abstract\nProline mediates metabolic communication between retinal pigment epithelial cells and the retina. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31110046/ · DOI 10.1074/jbc.RA119.007983","model_system":"Human RPE culture with pharmacological catabolism inhibition and metabolite measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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