{"id":"f6f52edb-439e-5d8e-bddc-b68661fa9e4a","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-scd-t10","predicate":"reduces","statement":"At 45 micromolar, the isomer reduced SCD activity in MDA-MB-231 and MCF-7 cells; protein fell only in MDA-MB-231, while mRNA was not repressed.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"80d80efa-81ce-53e5-b38b-a85af1b4744c","mechanism_event_label":"The same enzyme changed through different regulatory levels.","subject":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"object":{"id":"7bdb0604-dce4-5759-9331-7060cad57487","slug":"human-cell-scd-activity","display_name":"Human cell SCD activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"80d80efa-81ce-53e5-b38b-a85af1b4744c","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-scd-t10-event","event_type":"observed_relationship","label":"The same enzyme changed through different regulatory levels.","description":"At 45 micromolar, the isomer reduced SCD activity in MDA-MB-231 and MCF-7 cells; protein fell only in MDA-MB-231, while mRNA was not repressed.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7bdb0604-dce4-5759-9331-7060cad57487","slug":"human-cell-scd-activity","display_name":"Human cell SCD activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ff05052c-a102-5f63-bc9b-66a7a632ebf3","slug":"scd","display_name":"Human stearoyl-CoA desaturase / SCD","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human breast-cancer cell lines.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-line biochemistry does not establish anticancer benefit or ordinary dietary exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The same enzyme changed through different regulatory levels.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of stearoyl-CoA desaturase activity by the cis-9,trans-11 isomer and the trans-10,cis-12 isomer of conjugated linoleic acid in MDA-MB-231 and MCF-7 human breast cancer cells. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12061775/ · DOI 10.1016/S0006-291X(02)00554-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"168f437c-6776-5c5f-bb0b-1661006a0122","evidence_kind":"source_excerpt","locator":"Lines 62-68","start_line":62,"end_line":68,"excerpt":"## cla-scd-t10\nThe same enzyme changed through different regulatory levels.\nAt 45 micromolar, the isomer reduced SCD activity in MDA-MB-231 and MCF-7 cells; protein fell only in MDA-MB-231, while mRNA was not repressed.\nModel: Human breast-cancer cell lines.\nLimitations: Cell-line biochemistry does not establish anticancer benefit or ordinary dietary exposure.\nEvidence access: Primary abstract\nInhibition of stearoyl-CoA desaturase activity by the cis-9,trans-11 isomer and the trans-10,cis-12 isomer of conjugated linoleic acid in MDA-MB-231 and MCF-7 human breast cancer cells. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12061775/ · DOI 10.1016/S0006-291X(02)00554-5","model_system":"Human breast-cancer cell lines.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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