{"id":"f6d2ed9c-c517-503a-a670-ba580ac1c614","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:very-low-mg-suppresses-pth-in-human-cells","predicate":"depletion-suppresses","statement":"Lowering extracellular Mg to 0.1 mM suppressed PTH release from dispersed human parathyroid cells in the tested media.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"09244f11-2437-56ca-9a83-33a9b5212456","mechanism_event_label":"At very low Mg, the gland cells released less PTH even though simple loss of an inhibitory extracellular ion might predict more release.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"003d1a6f-e672-5a4e-b78b-fac58daaa987","slug":"pth-secretion","display_name":"Parathyroid hormone secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"09244f11-2437-56ca-9a83-33a9b5212456","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:very-low-mg-suppresses-pth-in-human-cells-event","event_type":"biochemical_relationship","label":"At very low Mg, the gland cells released less PTH even though simple loss of an inhibitory extracellular ion might predict more release.","description":"Lowering extracellular Mg to 0.1 mM suppressed PTH release from dispersed human parathyroid cells in the tested media.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"co-varied extracellular signal","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1c0d5c58-d358-5501-9bdf-8999bcf79fd4","slug":"casr","display_name":"Calcium-sensing receptor / CaSR","entity_type_key":"protein"},"role":"signaling receptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"003d1a6f-e672-5a4e-b78b-fac58daaa987","slug":"pth-secretion","display_name":"Parathyroid hormone secretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"magnesium -> CaSR/PTH -> calcium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dispersed human parathyroid cells from hyperparathyroid tissue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Experiment used hyperparathyroid tissue; culture concentrations are not clinical deficiency cutoffs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"At very low Mg, the gland cells released less PTH even though simple loss of an inhibitory extracellular ion might predict more release.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[quitterer-2001-magnesium-paradox] Paradoxical block of parathormone secretion is mediated by increased activity of G alpha subunits (2001). https://pubmed.ncbi.nlm.nih.gov/11102444/ DOI: 10.1074/jbc.M007727200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Parathyroid cells in vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"dc3a0cea-0ee6-5cde-9722-74edf79b15da","evidence_kind":"source_excerpt","locator":"Lines 409-419","start_line":409,"end_line":419,"excerpt":"### very-low-mg-suppresses-pth-in-human-cells\nLowering extracellular Mg to 0.1 mM suppressed PTH release from dispersed human parathyroid cells in the tested media.\nCondition category: nutrient_deficiency\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: At very low Mg, the gland cells released less PTH even though simple loss of an inhibitory extracellular ion might predict more release.\norganism: Homo sapiens\ntissue_or_cell_type: Parathyroid cells in vitro\nexperimental_model: Dispersed human parathyroid cells from hyperparathyroid tissue\nlimitations: Experiment used hyperparathyroid tissue; culture concentrations are not clinical deficiency cutoffs.\ncross_nutrient: magnesium -> CaSR/PTH -> calcium\n[quitterer-2001-magnesium-paradox] Paradoxical block of parathormone secretion is mediated by increased activity of G alpha subunits (2001). https://pubmed.ncbi.nlm.nih.gov/11102444/ DOI: 10.1074/jbc.M007727200","model_system":"Dispersed human parathyroid cells from hyperparathyroid tissue","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [quitterer-2001-magnesium-paradox] Paradoxical block of parathormone secretion is mediated by increased activity of G alpha subunits (2001). https://pubmed.ncbi.nlm.nih.gov/11102444/ DOI: 10.1074/jbc.M007727200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}