{"id":"f6bd2126-36ea-59ae-9648-6a28a3b41a93","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:mammalian-viability","predicate":"context_dependent_observation","statement":"Direct allicin exposure reduced mammalian-cell viability or proliferation in a concentration- and cell-type-dependent manner.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6f0659ee-9522-50c0-9623-561a464099a5","mechanism_event_label":"Direct allicin exposure reduced mammalian-cell viability or proliferation in a concentration- and cell-type-dependent manner.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"06e6a173-9bdd-56eb-b691-c9171af27fba","slug":"mammalian-allicin-viability-panel","display_name":"Viability of the 2017 mammalian allicin cell-line panel","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6f0659ee-9522-50c0-9623-561a464099a5","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:mammalian-viability-event","event_type":"experimental_observation","label":"Direct allicin exposure reduced mammalian-cell viability or proliferation in a concentration- and cell-type-dependent manner.","description":"**Mammalian injury is context-dependent.** Allicin reduced viability or proliferation and altered thiol-associated fluorescence in a panel including human A549, HUVEC, HT29, and MCF7 cells and mouse 3T3 cells. Sensitivity and apoptotic responses differed. Fluorescence changes are not a complete measurement of every glutathione species. Thiol reactivity does not guarantee selective toxicity to microbes or cancer cells. [Gruhlke 2017](https://pmc.ncbi.nlm.nih.gov/articles/PMC5384165/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Allicin concentration series","sequence_order":0,"notes":""},{"entity":{"id":"06e6a173-9bdd-56eb-b691-c9171af27fba","slug":"mammalian-allicin-viability-panel","display_name":"Viability of the 2017 mammalian allicin cell-line panel","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"mixed","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Allicin concentration series\", \"comparator\": \"Matched untreated cells in each model\", \"endpoint\": \"Direct allicin exposure reduced mammalian-cell viability or proliferation in a concentration- and cell-type-dependent manner.\", \"effect_direction\": \"mixed\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Human A549/HUVEC/HT29/MCF7 and mouse 3T3; separate viability, proliferation and apoptosis assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Separate species and endpoints; no cancer-selective clinical efficacy or direct measurement of every GSH species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Direct allicin exposure reduced mammalian-cell viability or proliferation in a concentration- and cell-type-dependent manner.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines. | 2016 | DOI 10.3390/antiox6010001 | PMID 28035949 | https://pubmed.ncbi.nlm.nih.gov/28035949/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC5384165/ | https://doi.org/10.3390/antiox6010001","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 53-53; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7446fbc9-3231-5396-a235-8c85a81bc1ee","evidence_kind":"source_excerpt","locator":"Lines 53-53","start_line":53,"end_line":53,"excerpt":"**Mammalian injury is context-dependent.** Allicin reduced viability or proliferation and altered thiol-associated fluorescence in a panel including human A549, HUVEC, HT29, and MCF7 cells and mouse 3T3 cells. Sensitivity and apoptotic responses differed. Fluorescence changes are not a complete measurement of every glutathione species. Thiol reactivity does not guarantee selective toxicity to microbes or cancer cells. [Gruhlke 2017](https://pmc.ncbi.nlm.nih.gov/articles/PMC5384165/)","model_system":"Human A549/HUVEC/HT29/MCF7 and mouse 3T3; separate viability, proliferation and apoptosis assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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