{"id":"f65d747e-c78c-55b7-a2c9-51c9583ed044","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-taucl-tnf","predicate":"reduces","statement":"Adding 0.5 mM taurine chloramine to activated RAW264.7 cells reduced TNF release by 43% in the reported assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fea81245-583c-5fee-8939-e0978e7573c2","mechanism_event_label":"A second inflammatory signal also fell.","subject":{"id":"0f99da38-0eae-53be-b1db-cb0146a11406","slug":"taurine-chloramine","display_name":"Taurine chloramine / TauCl","entity_type_key":"small_molecule"},"object":{"id":"df5e89d7-b1f8-5732-b730-b0bdd7b4f35d","slug":"mouse-taucl-macrophage-tnf","display_name":"TNF release from activated mouse RAW264.7 macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fea81245-583c-5fee-8939-e0978e7573c2","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-taucl-tnf-event","event_type":"observed_relationship","label":"A second inflammatory signal also fell.","description":"Adding 0.5 mM taurine chloramine to activated RAW264.7 cells reduced TNF release by 43% in the reported assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0f99da38-0eae-53be-b1db-cb0146a11406","slug":"taurine-chloramine","display_name":"Taurine chloramine / TauCl","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"df5e89d7-b1f8-5732-b730-b0bdd7b4f35d","slug":"mouse-taucl-macrophage-tnf","display_name":"TNF release from activated mouse RAW264.7 macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Same mouse-cell experiment as the nitric-oxide result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Two endpoints from one paper are not independent replications.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second inflammatory signal also fell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Taurine chloramine inhibits the synthesis of nitric oxide and the release of tumor necrosis factor in activated RAW 264.7 cells. · 1993 · https://pubmed.ncbi.nlm.nih.gov/7689627/ · DOI 10.1002/jlb.54.2.119","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"94241007-0570-5493-9731-4855e118cfb7","evidence_kind":"source_excerpt","locator":"Lines 377-383","start_line":377,"end_line":383,"excerpt":"## taurine-taucl-tnf\nA second inflammatory signal also fell.\nAdding 0.5 mM taurine chloramine to activated RAW264.7 cells reduced TNF release by 43% in the reported assay.\nModel: Same mouse-cell experiment as the nitric-oxide result.\nLimitations: Two endpoints from one paper are not independent replications.\nEvidence access: Primary abstract\nTaurine chloramine inhibits the synthesis of nitric oxide and the release of tumor necrosis factor in activated RAW 264.7 cells. · 1993 · https://pubmed.ncbi.nlm.nih.gov/7689627/ · DOI 10.1002/jlb.54.2.119","model_system":"Same mouse-cell experiment as the nitric-oxide result.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}