{"id":"f62e9036-1c0f-51a1-ab69-ed891c406204","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:apt2-kd-gpx4-mrna","predicate":"has_contextual_experimental_effect","statement":"APT2 knockdown did not significantly change GPX4 mRNA in the reported A375 and HT1080 RT-qPCR experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"a4f5cc16-fe54-503b-9c20-799ba07cbb04","mechanism_event_label":"apt2 kd gpx4 mrna","subject":{"id":"331ade05-c5c3-57df-85bc-bdffc00d531a","slug":"human-apt2-knockdown-a375","display_name":"Human APT2 knockdown in A375","entity_type_key":"protein_state"},"object":{"id":"8010f092-9f24-5fb1-8f83-ff45f316512c","slug":"human-gpx4-mrna-abundance","display_name":"GPX4 mRNA abundance in human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a4f5cc16-fe54-503b-9c20-799ba07cbb04","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:apt2-kd-gpx4-mrna","event_type":"experimental_result","label":"apt2 kd gpx4 mrna","description":"APT2 knockdown did not significantly change GPX4 mRNA in the reported A375 and HT1080 RT-qPCR experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"331ade05-c5c3-57df-85bc-bdffc00d531a","slug":"human-apt2-knockdown-a375","display_name":"Human APT2 knockdown in A375","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8010f092-9f24-5fb1-8f83-ff45f316512c","slug":"human-gpx4-mrna-abundance","display_name":"GPX4 mRNA abundance in human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"role":"base protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e9bb13ee-f238-51e6-9101-78ddfd11465c","slug":"gpx4","display_name":"GPX4","entity_type_key":"protein"},"role":"encoded protein","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round6-sources/gpx4-primary-passages.json; SHA256 384aaae29ec0e6fe9814782b3e5d885753751fa1b2bbd103e30e8ffa113df5dc","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human A375 and HT1080 cancer cells; lentiviral APT2 shRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"RT-qPCR; three independent experiments; exposure timing unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. The actor names the A375 arm; the parallel HT1080 observation is preserved in the context. No transcriptional effect is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human experimental cells; construct species unresolved where stated for discussion-only nulls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 5f","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-025-56344-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_access","value_text":"Selected primary Results and Figure 5/6 legends reviewed. No supplement or raw-data reanalysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"acd8aab0-7593-59a3-8e53-1efb41db9e63","evidence_kind":"source_excerpt","locator":"Lines 129-137","start_line":129,"end_line":137,"excerpt":"APT2 knockdown did not significantly change GPX4 mRNA in the reported A375 and HT1080 RT-qPCR experiments.\nprimary_references: https://doi.org/10.1038/s41467-025-56344-5\nprimary_locator: Figure 5f\nsource_access: Selected primary Results and Figure 5/6 legends reviewed. No supplement or raw-data reanalysis.\nevidence_cache: artifacts/discovery-research/round6-sources/gpx4-primary-passages.json; SHA256 384aaae29ec0e6fe9814782b3e5d885753751fa1b2bbd103e30e8ffa113df5dc\nexperimental_model: Human A375 and HT1080 cancer cells; lentiviral APT2 shRNA\norganism: Human experimental cells; construct species unresolved where stated for discussion-only nulls\nexposure: RT-qPCR; three independent experiments; exposure timing unresolved.\nlimitations: These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. The actor names the A375 arm; the parallel HT1080 observation is preserved in the context. No transcriptional effect is inferred.","model_system":"Human A375 and HT1080 cancer cells; lentiviral APT2 shRNA","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase and primary locator; not a verbatim publisher quote. Null-result limitations retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d7785cbf-e87c-5662-a76c-7ef11586cca5","stable_key":"import-e054f0f5-417f-5850-a874-91ff2c9de631","title":"APT2: sulforaphane engagement, GPX4 stability and ZDHHC6 turnover","document_type":"imported_text","citation_label":"Targeted primary-literature curation from recursive ZDHHC6 exploration, 2026-09-20.","file_path":"","sha256":"011acbeafd419a6826789806b59b5d7ff312b222762824580036325083586956","revision_id":"663a0bd1-3e46-5fee-ab0f-dcf992b2a809","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}