{"id":"f6139243-30cb-515f-b0a0-3d3f38eb3b67","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-glucose-acly-histones","predicate":"supports","statement":"Serum-induced histone acetylation in immortalized mouse embryonic fibroblasts depended on glucose availability and was prevented by Acly silencing.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e9fd10d4-0f8c-5a7d-98f5-e9062cefd7aa","mechanism_event_label":"Glucose supply affected histone acetylation through an acetyl-CoA-producing enzyme in this cell model.","subject":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"object":{"id":"ec8d4882-1462-5107-8400-367298f439e1","slug":"histone-acetylation","display_name":"Core histone lysine acetylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e9fd10d4-0f8c-5a7d-98f5-e9062cefd7aa","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-glucose-acly-histones-event","event_type":"biochemical_relationship","label":"Glucose supply affected histone acetylation through an acetyl-CoA-producing enzyme in this cell model.","description":"Serum-induced histone acetylation in immortalized mouse embryonic fibroblasts depended on glucose availability and was prevented by Acly silencing.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"283ce4c4-3ba6-5f26-93df-9939ef46d5fe","slug":"mouse-acly","display_name":"Mouse ATP-citrate lyase / Acly","entity_type_key":"protein"},"role":"required_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"acetyl_donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"44399a64-dd8a-5818-a863-b3389c6adf37","slug":"protein-bound-lysine","display_name":"Protein-bound lysine residue","entity_type_key":"protein_state"},"role":"protein_acceptor_residue","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ec8d4882-1462-5107-8400-367298f439e1","slug":"histone-acetylation","display_name":"Core histone lysine acetylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"BioC text line 21; Fig. 3B–C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Serum stimulation with/without glucose and Acly siRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Experimental serum stimulation and glucose withdrawal; no selective pantothenate deprivation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Glucose was the experimentally varied fuel. This is not evidence of B5 deficiency; cellular acetyl-CoA sources differ by context. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glucose supply affected histone acetylation through an acetyl-CoA-producing enzyme in this cell model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-chromatin2009] ATP-citrate lyase links cellular metabolism to histone acetylation. (2009). https://pubmed.ncbi.nlm.nih.gov/19461003/ DOI: 10.1126/science.1164097","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Immortalized mouse embryonic fibroblasts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4c1bd26c-d26e-5233-810e-1ed42cb5ed6d","evidence_kind":"source_excerpt","locator":"Lines 1019-1031","start_line":1019,"end_line":1031,"excerpt":"### b5-met-glucose-acly-histones\nSerum-induced histone acetylation in immortalized mouse embryonic fibroblasts depended on glucose availability and was prevented by Acly silencing.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Glucose supply affected histone acetylation through an acetyl-CoA-producing enzyme in this cell model.\norganism: Mus musculus\ntissue_or_cell_type: Immortalized mouse embryonic fibroblasts\nexperimental_model: Serum stimulation with/without glucose and Acly siRNA\nlimitations: Glucose was the experimentally varied fuel. This is not evidence of B5 deficiency; cellular acetyl-CoA sources differ by context. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Experimental serum stimulation and glucose withdrawal; no selective pantothenate deprivation.\ncross_nutrient: true\nevidence_location: BioC text line 21; Fig. 3B–C\n[b5-met-chromatin2009] ATP-citrate lyase links cellular metabolism to histone acetylation. (2009). https://pubmed.ncbi.nlm.nih.gov/19461003/ DOI: 10.1126/science.1164097","model_system":"Serum stimulation with/without glucose and Acly siRNA","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-chromatin2009] ATP-citrate lyase links cellular metabolism to histone acetylation. 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