{"id":"f55beaf0-bd78-50b0-a2ba-c73fc7ad1bda","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-fdx2-iron","predicate":"contains","statement":"Purified reconstructed FDX2 retained a two-iron, two-sulfur cluster.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3b6d83a2-3abd-5aff-aa0d-354ebdfd3194","mechanism_event_label":"Iron participates in an electron-transfer component, not as a substitute for CoQ.","subject":{"id":"0c81d159-49cc-5be8-996e-7cee8944d794","slug":"tet-fdx2","display_name":"Reconstructed ancestral tetrapod FDX2","entity_type_key":"protein"},"object":{"id":"02107865-f169-5624-9ea4-9448a4cce185","slug":"iron-sulfur-2fe2s","display_name":"[2Fe-2S] iron-sulfur cluster","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"3b6d83a2-3abd-5aff-aa0d-354ebdfd3194","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-fdx2-iron-event","event_type":"biochemical_relationship","label":"Iron participates in an electron-transfer component, not as a substitute for CoQ.","description":"Purified reconstructed FDX2 retained a two-iron, two-sulfur cluster.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0c81d159-49cc-5be8-996e-7cee8944d794","slug":"tet-fdx2","display_name":"Reconstructed ancestral tetrapod FDX2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"02107865-f169-5624-9ea4-9448a4cce185","slug":"iron-sulfur-2fe2s","display_name":"[2Fe-2S] iron-sulfur cluster","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 9067, \"end_char\": 13813, \"text_sha256\": \"49612ae4d3a59a75a02fe3fb132abd8aeb7ef8b7cae3b0f79b5557503c55ad51\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified reconstructed COQ metabolon with short-chain substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enzyme combinations, methyl donors, reductants and metal additions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. 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(2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stepwise CoQ head-group assembly","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eb2867bd-b939-53a2-918e-dfc917ac99a4","evidence_kind":"source_excerpt","locator":"Lines 606-617","start_line":606,"end_line":617,"excerpt":"### coq10-fdx2-iron\nPurified reconstructed FDX2 retained a two-iron, two-sulfur cluster.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Iron participates in an electron-transfer component, not as a substitute for CoQ.\norganism: Reconstructed ancestral tetrapod proteins\ntissue_or_cell_type: Stepwise CoQ head-group assembly\nexperimental_model: Purified reconstructed COQ metabolon with short-chain substrates\nlimitations: Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.\nexposure: Enzyme combinations, methyl donors, reductants and metal additions\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 9067, \"end_char\": 13813, \"text_sha256\": \"49612ae4d3a59a75a02fe3fb132abd8aeb7ef8b7cae3b0f79b5557503c55ad51\"}\n[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","model_system":"Purified reconstructed COQ metabolon with short-chain substrates","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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