{"id":"f5555284-bb7e-591a-93f3-045f8be6d793","stable_key":"6660bba2-4fa0-590a-bf52-9803a24bea9f:dat-knockout","predicate":"decreases_in_recorded_experiment","statement":"DAT knockout abolished the usual modafinil wake-promoting response in mice while caffeine remained effective with enhanced response.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e9d9edcf-2141-56bd-bc06-95cf241a06b6","mechanism_event_label":"DAT knockout abolished the usual modafinil wake-promoting response in mice while caffeine remained effective with enhanced response.","subject":{"id":"ad24f800-be90-522c-8895-21a7555858f2","slug":"mouse-slc6a3","display_name":"Mouse dopamine transporter / Slc6a3","entity_type_key":"protein"},"object":{"id":"2783faf9-3c82-5360-8540-e30f0f7310a1","slug":"mouse-wake-duration","display_name":"Mouse wake duration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e9d9edcf-2141-56bd-bc06-95cf241a06b6","stable_key":"6660bba2-4fa0-590a-bf52-9803a24bea9f:dat-knockout-event","event_type":"experimental_observation","label":"DAT knockout abolished the usual modafinil wake-promoting response in mice while caffeine remained effective with enhanced response.","description":"In DAT-knockout mice, modafinil lost its usual wake-promoting effect; caffeine remained effective and produced an enhanced response. This supports a DAT-dependent route in that model, rather than a general inability of those mice to awaken. Developmental compensation and changed baseline physiology still limit extrapolation from a constitutive knockout. [Wisor et al., 2001](https://pubmed.ncbi.nlm.nih.gov/11222668/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ad24f800-be90-522c-8895-21a7555858f2","slug":"mouse-slc6a3","display_name":"Mouse dopamine transporter / Slc6a3","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"Modafinil in DAT-knockout mice","sequence_order":0,"notes":""},{"entity":{"id":"2783faf9-3c82-5360-8540-e30f0f7310a1","slug":"mouse-wake-duration","display_name":"Mouse wake duration","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"624e6a4b-f96d-5d12-8206-30d1e1715c26","slug":"modafinil","display_name":"Modafinil","entity_type_key":"drug"},"role":"drug challenge","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ef028472-7c33-583d-b52e-40676004d370","slug":"caffeine","display_name":"Caffeine","entity_type_key":"small_molecule"},"role":"active comparative drug","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract checked; full methods and figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"drug challenge","comparator":"Modafinil in wild-type mice","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"modafinil","display_name":"Modafinil","entity_type_key":"drug"}},{"dimension":"experimental_condition","value_text":"knockout","comparator":"Modafinil in wild-type mice","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"mouse-slc6a3","display_name":"Mouse dopamine transporter / Slc6a3","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Modafinil in DAT-knockout mice\", \"comparator\": \"Modafinil in wild-type mice\", \"endpoint\": \"DAT knockout abolished the usual modafinil wake-promoting response in mice while caffeine remained effective with enhanced response.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"mouse-slc6a3\", \"state\": \"knockout\"}, {\"entity_slug\": \"modafinil\", \"state\": \"drug challenge\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Constitutive mouse DAT knockout; baseline and developmental compensation constrain interpretation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. 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