{"id":"f53f57d8-f917-5301-b74a-63ef5233d23c","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-10-5ht3-flux","predicate":"inhibits","statement":"10-Gingerol inhibited 5-HT3-mediated cation influx in N1E-115 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"fd1aa56b-3494-52aa-924a-c0de4f2468e5","mechanism_event_label":"10-Gingerol inhibited 5-HT3-mediated cation influx in N1E-115 cells.","subject":{"id":"bf4fa054-0b02-5fc2-82dd-2e53b36033b3","slug":"10-gingerol","display_name":"10-Gingerol","entity_type_key":"small_molecule"},"object":{"id":"b848e40a-7182-5531-a7f4-28dbea723f10","slug":"mouse-5ht3-receptor-complex","display_name":"Mouse 5-HT3 receptor-channel complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"fd1aa56b-3494-52aa-924a-c0de4f2468e5","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-10-5ht3-flux-event","event_type":"observed_relationship","label":"10-Gingerol inhibited 5-HT3-mediated cation influx in N1E-115 cells.","description":"10-Gingerol inhibited 5-HT3-mediated cation influx in N1E-115 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bf4fa054-0b02-5fc2-82dd-2e53b36033b3","slug":"10-gingerol","display_name":"10-Gingerol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b848e40a-7182-5531-a7f4-28dbea723f10","slug":"mouse-5ht3-receptor-complex","display_name":"Mouse 5-HT3 receptor-channel complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fef9ecc3-7c04-553c-a207-d91e38a2665a","slug":"serotonin","display_name":"Serotonin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Concentration-dependent gingerol application; exact range not in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse N1E-115 neuroblastoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cation flux is a functional assay, not a demonstrated orthosteric binding site or clinical antiemetic response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse N1E-115 neuroblastoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"10-Gingerol inhibited 5-HT3-mediated cation influx in N1E-115 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Native 5-HT3 receptor-channel complex","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cfd378f3-9663-549b-b161-cdc7909f5104","evidence_kind":"source_excerpt","locator":"Lines 118-127","start_line":118,"end_line":127,"excerpt":"## gingerols-10-5ht3-flux\n10-Gingerol inhibited 5-HT3-mediated cation influx in N1E-115 cells.\nModel/species: Mouse N1E-115 neuroblastoma cells\nTissue: Native 5-HT3 receptor-channel complex\nExposure: Concentration-dependent gingerol application; exact range not in accessed abstract\nRoute: In vitro addition\nDuration: Not specified in accessed abstract\nLimits: Cation flux is a functional assay, not a demonstrated orthosteric binding site or clinical antiemetic response.\nPrimary reference: Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049\nAccess: Primary PubMed abstract.","model_system":"Mouse N1E-115 neuroblastoma cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived synthesis span with its primary citation; original paraphrase, not a claimed publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1107d60b-8e2d-55d7-8f5d-5612c49aa7dc","stable_key":"import-fd7915ff-856d-56c2-a118-4989693a7644","title":"Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text.","file_path":"","sha256":"96d1b7b04445eecd0eac1738e0c80cb6308509ecdfc3b5fc6be67ae2218cbb2b","revision_id":"de6e70ff-0e52-5c26-bd3a-54fca2085a86","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}