{"id":"f5362bea-4ab6-5106-ac61-2a12a52d96a5","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-protein-use","predicate":"increases","statement":"Proteolysis and protein oxidation increased approximately 50%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fe74eb33-a16a-5ae1-b302-d52b6c8562f5","mechanism_event_label":"This protocol did not preserve all body protein.","subject":{"id":"2d5df4b9-44d7-56a5-8ecf-7c08c0ae9f4f","slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"},"object":{"id":"5367b999-9289-5d10-aae1-3a0495ee9076","slug":"whole-body-protein-oxidation","display_name":"Whole-body protein oxidation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fe74eb33-a16a-5ae1-b302-d52b6c8562f5","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-protein-use-event","event_type":"observed_relationship","label":"This protocol did not preserve all body protein.","description":"Proteolysis and protein oxidation increased approximately 50%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2d5df4b9-44d7-56a5-8ecf-7c08c0ae9f4f","slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5367b999-9289-5d10-aae1-3a0495ee9076","slug":"whole-body-protein-oxidation","display_name":"Whole-body protein oxidation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Six men; isotope tracers and indirect calorimetry during a 60-hour fast.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Protocol-specific fluxes; no universal hour-by-hour threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"This protocol did not preserve all body protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fuel and energy metabolism in fasting humans. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8017334/ · DOI 10.1093/ajcn/60.1.29","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a13c8e09-2804-599c-bbcf-b6aab357ea94","evidence_kind":"source_excerpt","locator":"Lines 56-62","start_line":56,"end_line":62,"excerpt":"## fast-protein-use\nThis protocol did not preserve all body protein.\nProteolysis and protein oxidation increased approximately 50%.\nModel: Six men; isotope tracers and indirect calorimetry during a 60-hour fast.\nLimitations: Protocol-specific fluxes; no universal hour-by-hour threshold.\nEvidence access: Primary abstract\nFuel and energy metabolism in fasting humans. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8017334/ · DOI 10.1093/ajcn/60.1.29","model_system":"Six men; isotope tracers and indirect calorimetry during a 60-hour fast.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"921d1eb5-2c88-52b9-9898-60b2b5bf6029","stable_key":"import-c9aa15a1-8ba2-5913-b2dd-372af304bf87","title":"Fasting: fuel switching, nutrient sensing, ketone signaling, nutrient dependencies and refeeding (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"991b7fd94be84c0f1e622e0672eda03d600b388dfd7825e219aa1678d1c7983a","revision_id":"ce85d3f4-4eaf-5aee-bddb-0f0ce7fe2211","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}