{"id":"f4b16bbb-93f0-547c-9227-3814ab5448fa","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-glut1-expression","predicate":"reduces_tested_expression_of","statement":"Cucurbitacin D decreased GLUT1 expression in the prostate-cancer study; miR-132-associated changes were also reported.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"dd2aae32-e5a9-5d9e-82a4-49b3f0c88088","mechanism_event_label":"Transporter abundance offers one route to altered glucose handling.","subject":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"object":{"id":"ffb5e632-9a8e-5bed-97eb-56a9ecdd856f","slug":"slc2a1","display_name":"Human glucose transporter 1 / SLC2A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"dd2aae32-e5a9-5d9e-82a4-49b3f0c88088","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-glut1-expression-event","event_type":"observed_relationship","label":"Transporter abundance offers one route to altered glucose handling.","description":"Cucurbitacin D decreased GLUT1 expression in the prostate-cancer study; miR-132-associated changes were also reported.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ffb5e632-9a8e-5bed-97eb-56a9ecdd856f","slug":"slc2a1","display_name":"Human glucose transporter 1 / SLC2A1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e5718caf-534d-5feb-a240-038eb7baa501","slug":"human-prostate-cud-glucose-uptake","display_name":"Human prostate-cancer glucose uptake during cucurbitacin D exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human PC3/DU145 cells and xenograft work.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression and correlated microRNA changes do not establish a single exclusive mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Transporter abundance offers one route to altered glucose handling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin D Reprograms Glucose Metabolic Network in Prostate Cancer. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30875788/ · DOI 10.3390/cancers11030364","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"38ae897a-f279-5788-a04a-eded530264b0","evidence_kind":"source_excerpt","locator":"Lines 316-322","start_line":316,"end_line":322,"excerpt":"## cucurbitacin-d-glut1-expression\nTransporter abundance offers one route to altered glucose handling.\nCucurbitacin D decreased GLUT1 expression in the prostate-cancer study; miR-132-associated changes were also reported.\nModel: Human PC3/DU145 cells and xenograft work.\nLimitations: Expression and correlated microRNA changes do not establish a single exclusive mechanism.\nEvidence access: Primary abstract\nCucurbitacin D Reprograms Glucose Metabolic Network in Prostate Cancer. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30875788/ · DOI 10.3390/cancers11030364","model_system":"Human PC3/DU145 cells and xenograft work.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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