{"id":"f484f50b-ebb3-580f-a8d0-62ed5266c7e9","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-liver-cyp3a4-null","predicate":"had_little_effect_on_in_liver_slices","statement":"The same liver-slice experiment found little effect of DIM on CYP3A4 protein.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ac19091d-2e86-5905-bf5d-29a1f530059b","mechanism_event_label":"A result for one CYP family cannot simply be assigned to another.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"54e95ff1-4ffa-5e6f-a4cb-b47e5d2a5bda","slug":"cyp3a4","display_name":"Human cytochrome P450 3A4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ac19091d-2e86-5905-bf5d-29a1f530059b","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-liver-cyp3a4-null-event","event_type":"biochemical_relationship","label":"A result for one CYP family cannot simply be assigned to another.","description":"The same liver-slice experiment found little effect of DIM on CYP3A4 protein.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"54e95ff1-4ffa-5e6f-a4cb-b47e5d2a5bda","slug":"cyp3a4","display_name":"Human cytochrome P450 3A4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/9741959.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\", \"start_char\": 0, \"end_char\": 1338, \"text_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Precision-cut liver-slice induction and immunoblotting","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM 50 micromolar for 72 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex-vivo exposure; not a measured oral DIM effect on a patient drug concentration. Four donors showed variable responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human donor liver slices","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A result for one CYP family cannot simply be assigned to another.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p9741959] 3,3'-Diindolylmethane induces CYP1A2 in cultured precision-cut human liver slices. (1998). https://pubmed.ncbi.nlm.nih.gov/9741959/ DOI: 10.1080/004982598239227","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"CYP1A proteins and catalytic assays","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3c73c7a6-2442-5e94-9944-dfed2a66b96e","evidence_kind":"source_excerpt","locator":"Lines 363-374","start_line":363,"end_line":374,"excerpt":"### dim-liver-cyp3a4-null\nThe same liver-slice experiment found little effect of DIM on CYP3A4 protein.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A result for one CYP family cannot simply be assigned to another.\norganism: Human donor liver slices\ntissue_or_cell_type: CYP1A proteins and catalytic assays\nexperimental_model: Precision-cut liver-slice induction and immunoblotting\nlimitations: Ex-vivo exposure; not a measured oral DIM effect on a patient drug concentration. Four donors showed variable responses.\nexposure: DIM 50 micromolar for 72 hours\nevidence_span: {\"source_cache\": \"artifacts/dim-research/9741959.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\", \"start_char\": 0, \"end_char\": 1338, \"text_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\"}\n[dim-p9741959] 3,3'-Diindolylmethane induces CYP1A2 in cultured precision-cut human liver slices. (1998). https://pubmed.ncbi.nlm.nih.gov/9741959/ DOI: 10.1080/004982598239227","model_system":"Precision-cut liver-slice induction and immunoblotting","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dim-p9741959] 3,3'-Diindolylmethane induces CYP1A2 in cultured precision-cut human liver slices. (1998). https://pubmed.ncbi.nlm.nih.gov/9741959/ DOI: 10.1080/004982598239227","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b6d70682-97f9-5893-a03c-f9f88836033c","stable_key":"import-182336c6-ed36-5ec6-8a09-25c31096262e","title":"Diindolylmethane (DIM): formation, receptor signaling, metabolism and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"31e14060de904976d2f5d439f2bc9c8a99a72d34626c562c78429025e050d404","revision_id":"611094c1-a9cf-5b9b-b255-ac70b571696a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}