{"id":"f47c3311-c839-5579-ab74-abd4dcbbf468","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-wnk1-binding","predicate":"binds","statement":"Chloride bound the WNK1 catalytic region and stabilized its inactive conformation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e342756b-2958-59ba-8a1d-bf99e46bfec1","mechanism_event_label":"Chloride can act as a direct signal to a kinase, not just as an accompanying ion.","subject":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"object":{"id":"ba9aabe0-e3e5-5b4e-b4fc-be0d6b53bb7c","slug":"wnk1","display_name":"Human WNK lysine deficient protein kinase 1 / WNK1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e342756b-2958-59ba-8a1d-bf99e46bfec1","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-wnk1-binding-event","event_type":"biochemical_relationship","label":"Chloride can act as a direct signal to a kinase, not just as an accompanying ion.","description":"Chloride bound the WNK1 catalytic region and stabilized its inactive conformation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ba9aabe0-e3e5-5b4e-b4fc-be0d6b53bb7c","slug":"wnk1","display_name":"Human WNK lysine deficient protein kinase 1 / WNK1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/24803536.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"77c3ca2b36fc42c41bf361696bca69b3c185442ff3ef389055a95f4615dda6cb\", \"start_char\": 0, \"end_char\": 1200, \"text_sha256\": \"77c3ca2b36fc42c41bf361696bca69b3c185442ff3ef389055a95f4615dda6cb\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystallography and kinase mutagenesis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chloride binding and chloride-pocket mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-free sensing; plasma chloride is not identical to kinase-site chloride.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human WNK1 kinase constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Chloride can act as a direct signal to a kinase, not just as an accompanying ion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p24803536] Chloride sensing by WNK1 involves inhibition of autophosphorylation. 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