{"id":"f43eb704-4f18-559f-a06e-7526e0056d08","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-nav15","predicate":"inhibits","statement":"Eugenol concentration-dependently inhibited human Nav1.5, favored the inactivated state, slowed recovery, and attenuated late sodium current.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ffbf0a66-e7ec-5023-b488-601f8a1e310d","mechanism_event_label":"Eugenol concentration-dependently inhibited human Nav1.5, favored the inactivated state, slowed recovery, and attenuated late sodium current.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"f728754d-bc3b-560f-8077-96193e04d162","slug":"human-nav15-current","display_name":"Human cardiac Nav1.5 sodium current","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ffbf0a66-e7ec-5023-b488-601f8a1e310d","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-nav15-event","event_type":"observed_relationship","label":"Eugenol concentration-dependently inhibited human Nav1.5, favored the inactivated state, slowed recovery, and attenuated late sodium current.","description":"Eugenol concentration-dependently inhibited human Nav1.5, favored the inactivated state, slowed recovery, and attenuated late sodium current.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f728754d-bc3b-560f-8077-96193e04d162","slug":"human-nav15-current","display_name":"Human cardiac Nav1.5 sodium current","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0ce76706-9da7-5d51-a91b-dd7e2e1391a4","slug":"scn5a","display_name":"Human cardiac sodium channel Nav1.5 / SCN5A","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol concentration-response; ouabain arrhythmia model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HEK cells expressing human Nav1.5; isolated guinea-pig atria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Channel inhibition can alter cardiac excitability; isolated atrial antiarrhythmic effects are not a human treatment result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"HEK cells expressing human Nav1.5; isolated guinea-pig atria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Eugenol concentration-dependently inhibited human Nav1.5, favored the inactivated state, slowed recovery, and attenuated late sodium current.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Eugenol interacts with cardiac sodium channel and reduces heart excitability and arrhythmias. (2021). https://pubmed.ncbi.nlm.nih.gov/34217764/ DOI: 10.1016/j.lfs.2021.119761","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro and ex vivo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Cardiac sodium current and ex-vivo rhythm disturbance","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a4dff013-796a-530a-a8a6-1820f30e4b8e","evidence_kind":"source_excerpt","locator":"Lines 55-64","start_line":55,"end_line":64,"excerpt":"## eugenol-nav15\nEugenol concentration-dependently inhibited human Nav1.5, favored the inactivated state, slowed recovery, and attenuated late sodium current.\nModel/species: HEK cells expressing human Nav1.5; isolated guinea-pig atria\nTissue/system: Cardiac sodium current and ex-vivo rhythm disturbance\nExposure: Eugenol concentration-response; ouabain arrhythmia model\nRoute: In vitro and ex vivo\nDuration: Acute\nLimits: Channel inhibition can alter cardiac excitability; isolated atrial antiarrhythmic effects are not a human treatment result.\nPrimary reference: Eugenol interacts with cardiac sodium channel and reduces heart excitability and arrhythmias. (2021). https://pubmed.ncbi.nlm.nih.gov/34217764/ DOI: 10.1016/j.lfs.2021.119761\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"HEK cells expressing human Nav1.5; isolated guinea-pig atria","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}